芦笋种质资源的SRAP遗传多样性分析  

Genetic Diversity Analysis of Asparagus Based on SRAP Markers

作　　者：李霞[1] 李书华[1] 杨林[1] 于继庆[1] 李保华[1] 郑鑫[1] 包艳存 

机构地区：[1]山东省潍坊市农业科学院,山东潍坊261071

出　　处：《农学学报》2016年第3期40-45,共6页Journal of Agriculture

基　　金：山东省科技发展计划专项计划项目“芦笋超雄株技术研究与示范”(2012GZX21024)

摘　　要：为了更深入地揭示芦笋品种（系）的遗传多样性,本研究采用SRAP技术对国内外12个芦笋栽培种和杂交种的遗传多态性进行分析。结果表明,从170对引物中筛选出29对多态性强、条带清晰的引物,每对引物产生12~36条谱带,共产生551条谱带,其中多态性条带343条,占总带数的61.21%。平均每个引物扩增的DNA带数是20.41条,多态性带数是12.7条。采用NTSYSpc2.1软件对SRAP扩增结果进行聚类分析,12份芦笋材料的相似系数在0.39~0.97之间,平均相似系数为0.69。在Coefficient=0.69处划等值线,可将12份芦笋材料划分为4类。第一类：‘鲁芦笋一号’、‘88-5’、‘格兰德’、‘07-1’和‘06-6’。第二类：‘阿波罗’、‘泽西巨人’和‘阿特拉斯’。第三类：‘西班牙达玛斯’。第四类：‘荷兰全雄’、‘UC800’和‘西德全雄’。This paper analyzed the genetic polymorphism of 12 asparagus cultivars and hybrids from home andabroad using SRAP method, so as to deeply reveal the genetic diversity of asparagus varieties. The resultsshowed that 29 primers with high polymorphism and clear bands were screened from 170 primers, each of themamplified 12 to 36 bands and a total of 551 DNA fragments were obtained, 343 bands showed polymorphism inall fragments, accounted for 61.21%. The average number of DNA bands produced by each primer was 20.41 and polymorphic bands were 12.7. The result of genetic similarity analysis of 12 asparagus genotypes showedthat the Nei＇s coefficient ranged from 0.39 to 0.97, and the average Nei＇s coefficient was 0.69. A DNAmolecular dendrogram was established for 12 genotypes which were divided into 4 groups according to thecoefficient of 0.69. The first group included‘Lu Asparagus No.1＇,‘88-5＇,‘Gland＇,‘07-1＇and‘06-6＇.The second group included‘Apollo＇,‘Jersey Giant＇and‘Atlas＇. The third group included‘Spanish Damas＇.The forth group included‘Holland all male＇and‘West Germany all male＇.

关 键 词：芦笋 种质资源 SRAP 遗传多样性 

分 类 号：S644.6[农业科学—蔬菜学]