薏苡仁酯对胶原诱导性关节炎小鼠Foxp3^+ CD4^+ CD25^+调节性T细胞影响的研究  

Effect of Coixenolide on Foxp3^+ CD4^+ CD25^+ Regulatory T Cells in Collagen-induced Arthritis Mice

作　　者：郑红霞[1] 章伟明[2] 周红娟[1] 张雯[1] 于健宁[1] 汪卫[1] 

机构地区：[1]浙江省中西医结合医院风湿免疫科,杭州310003 [2]杭州市第一人民医院药剂科,杭州310009

出　　处：《中国中西医结合杂志》2016年第3期348-350,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：浙江省中医药管理局资助课题(No.2011ZB106)

摘　　要：目的研究薏苡仁酯对胶原诱导性关节炎(collagen induced arthritis,CIA)小鼠外周血中Foxp3+CD4+CD25+调节性T细胞(Treg)的影响,探讨薏苡仁酯类风湿关节炎治疗中的可能机制。方法从25只小鼠随机选取5只为正常组,剩余20只小鼠进行CIA造模。造模成功后随机分为造模对照组10只及薏苡仁酯组10只。薏苡仁酯组予薏苡仁酯注射液25 m L/kg,正常组及造模对照组注射生理盐水25 m L/kg,均每天腹腔内注射1次。共用药21天。用药后对各组关节炎进行评分,计算关节炎指数,并应用流式细胞术测定小鼠外周血Foxp3+CD4+CD25+Treg比例。结果与正常组比较,造模对照组关节炎指数明显升高(P<0.01),薏苡仁酯组较造模对照组小鼠关节炎指数明显下降(P<0.01)。与正常组比较,造模对照组Foxp3+CD4+CD25+Treg水平均明显下降(P<0.01),薏苡仁酯组较造模对照组用药后Foxp3+CD4+CD25+Treg水平明显升高,差异有统计学意义(P<0.01)。结论薏苡仁酯能上调CIA小鼠Foxp3+CD4+CD25+Treg比例,可能在CIA的发病中具有一定的免疫调节作用。Objective To study the effect of coixenolide on Foxp3＋CD4＋CD25＋regulatory T cells（ Treg） in collagen induced arthritis（ CIA） mice,and to explore its possible mechanism for treating rheumatiol arthritis. Methods Five mice were recruited as a normal control group from 25 mice,and the rest 20 were used in CIA modeling. After successful modeling they were randomly divided in the model control group and the coixenolide group,10 in each group. Coixenolide injection at 25 m L / kg was intraperitoneally injected to mice in the coixenolide group,while normal saline at 25 m L / kg was intraperitoneally injected to mice in the normal control group and the model control group. The injection lasted for 21 days.Scoring for CIA was performed after injection and arthritis index was calculated. The peripheral blood Foxp3＋CD4＋CD25＋Treg ratio was determined by flow cytometry（ FCM）. Results Compared with the normal control group,the arthritis index obviously increased in the model control group（P 〈0. 01）. The arthritis index obviously decreased more in the coixenolide group than in the model control group（P 〈0. 01）. Foxp3＋CD4＋CD25＋Treg levels obviously decreased more in the model control group than in the normal control group（P 〈0. 01）. Foxp3＋CD4＋CD25＋Treg levels obviously increased more in the coixenolide control group than in the model control group（P〈 0. 01）. Conclusion Coixenolide could up-regulate Foxp3＋CD4＋CD25＋Treg ratios in CIA mice,which might play certain immunoregulation roles in the incidence of CIA.

关 键 词：胶原诱导性关节炎 薏苡仁酯 Foxp3+ CD4+ CD25+ Treg 

分 类 号：R285.5[医药卫生—中药学]