36种中草药提取物对烟草赤星病菌的抑制作用  

The Inhibitory Effects Thirty-six Herbal Medicine Extracts against Alternaria alternate

作　　者：袁圆[1] 桑维钧[2] 石春发[1] 刁朝强 王德凤[1] 刘宁国[1] 崔庆彩 

机构地区：[1]贵州大学农学院,贵阳550025 [2]贵州大学烟草学院,贵阳550025 [3]贵州省烟草公司贵阳市分公司,贵阳550001

出　　处：《湖北农业科学》2016年第5期1159-1162,共4页Hubei Agricultural Sciences

基　　金：贵州省烟草公司项目(2013-2015)

摘　　要：对36种中草药分别进行微波法提取及水蒸气蒸馏法提取,以烟草赤星病菌(Alternaria alternate)为供试菌种,采用菌丝生长速率法对其提取物进行抑菌作用研究。结果表明,两种方法的提取物对烟草赤星病菌基本都有抑制作用。其中,在微波法中,黄连和雄黄的提取物初步抑制作用最好,抑制率分别为91.15%和76.72%,桑寄生、甘草和白果在60%以上。在室内毒力测定研究中,黄连和雄黄的EC50分别为7.077 3 mg/m L和140.651 3 mg/m L。在水蒸气蒸馏法中,抑菌作用最强的是大黄、七叶一枝花和肉桂,抑制率均在60%以上,分别为69.93%、68.14%和63.07%。36 kinds of herbs were respectively extracted with microwave and steam distillation, the inhibition of which was carried out by mycelial growth rate method with Alternaria alternate as the test object. The results showed that the extracts of the two methods had inhibitory effects on A.Alternate. The best preliminary inhibitory effects were rhizoma coptidis and realgar of the microwave method, their inhibition rate were 91.15% and 76.72% respectively, the mulberry parasitism, liquorice and gingko were above 60%. In the study of indoor toxicity measurement,EC50 of rhizoma coptidis and realgar were 7.077 3 mg / m L and 140.651 3 mg / m L. In the water vapour distillation, the strongest inhibitions were rhubarb, paris polyphylla and cinnamon,whose inhibition rate were over 60%,69.93%,68.14% and 63.07% respectively.

关 键 词：中草药 提取物 烟草赤星病菌(Alternaria alternate) 

分 类 号：S432.44[农业科学—植物病理学]