全视网膜激光光凝对增生型糖尿病视网膜病变视网膜前膜中环氧化酶-2、血管内皮生长因子表达的影响  

Effect of panretinal photocoagulation on the expression of cyclooxygenase-2, vascular endothelial cell growth factor in epiretinal membrane of proliferative diabetic retinopathy

作　　者：韩林峰[1] 柯根杰[1] 王林[1] 顾永昊[1] 顾起宏[1] 董凯[1] 刘家佳[1] 

机构地区：[1]安徽医科大学附属省立医院眼科,合肥230001

出　　处：《中华眼底病杂志》2016年第2期140-143,共4页Chinese Journal of Ocular Fundus Diseases

基　　金：国家自然科学基金（81400407）;安徽省科技厅科研计划项目（15011d04025）

摘　　要：目的观察全视网膜激光光凝（PRP）对增生型糖尿病视网膜病变（PDR）患者视网膜前膜组织中环氧化酶-2（COX-2）、血管内皮生长因子（VEGF）表达的影响。方法临床检查确诊并接受玻璃体切割手术治疗的PDR患者35例35只眼纳入研究。根据手术前是否接受过PRP治疗将患者分为非光凝组和光凝组，分别为19例19只眼和16例16只眼。收集行玻璃体切割手术剥离的视网膜前膜病理标本，苏木精-伊红染色光学显微镜观察其组织病理特征；CD34单克隆抗体、COX-2单克隆抗体、VEGF单克隆抗体、链霉菌抗生物素蛋白-过氧化物酶连结法免疫组织化学方法测定视网膜前膜微血管密度（MVD）和COX-2、VEGF表达。结果非光凝组患眼视网膜前膜大而肥厚，纤维组织结构内可见数量较多、管径较大的新生血管；光凝组患眼视网膜前膜相对较薄，可见散在、管径较小的新生血管。非光凝组、光凝组患眼视网膜前膜平均MVD分别为7．42±1．39、4．56±1．22；非光凝组患眼MVD数量明显高于光凝组，差异有统计学意义（t=6．41，P〈0．001）。非光凝组患眼视网膜前膜CD34（t=6．147）、COX-2（t=5．944）、VEGF（t=7．445）表达明显高于光凝组，差异均有统计学意义（P〈0．001）。结论PRP能有效抑制PDR视网膜前膜中COX-2、VEGF表达。Objective To observe the effect of panretinal photocoagulation （PRP） on the expression of cyclooxygenase-2 （COX-2）, vascular endothelial cell growth factor （VEGF） in epiretinal membrane of proliferative diabetic retinopathy （PDR）. Methods A total of 35 patients （35 eyes） with PDR and underwent plana vitrectomy were enrolled in this study. The patients were divided into non-PRP group （19 patients, 19 eyes） and PRP group （16 patients, 16 eyes） depends on if they had received PRP before surgery. The epiretinal membranes stripped during operation were collected for pathological examination. The histopathological features was observed by haematoxylin and eosin stain. The expression of CD34, COX-2 and VEGF, and microvessel density （MVD） were measured by immunohistochemistry method. Results Many new dispersed capillary blood vessels were found in the thick epiretinal membranes of non- PRP group, while scattered Small blood vessels were found in the relatively thin epiretinal membranes of PRP group. MVD value was （7.42 ± 1.39） in the non-PRP group and （4.56 ± 1.22） in the PRP group, which was lower than the non-PRP group （t=6.41, P〈0.01）. The expression of CD34, COX-2 and VEGF in the tissues of epiretinal membrane in PRP group were obviously lower than the non-PRP group （t= 6. 147, 5. 944, 7. 445; P〈0. 01）. Conclusion PRP can effectively inhibit the expression of COX-2 and VEGF in epiretinal membrane of PRP patients.

关 键 词：糖尿病视网膜病变/治疗 激光凝固术/副作用 环氧化酶2 血管内皮生长因子类 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]