茯苓对裸鼠SGC-7901胃癌细胞移植瘤的影响  

Effect of Poria Cocos on Xenograft Tumor of Human Gastric Cancer SGC-7901 Cell Line in Nude Mice

作　　者：刘元[1,2] 钱军[1] 郭晨旭[1] 承泽农[3] 

机构地区：[1]蚌埠医学院第一附属医院肿瘤外三科,安徽蚌埠233000 [2]蚌埠医学院研究生部,安徽蚌埠233000 [3]蚌埠医学院第一附属医院病理科,安徽蚌埠233000

出　　处：《中国普外基础与临床杂志》2016年第3期286-291,共6页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金资助项目(项目编号:81173386)~~

摘　　要：目的研究中药茯苓对SGC-7901胃癌细胞株裸鼠体内移植瘤的影响。方法 1将10只裸鼠建立移植瘤模型后,随机分为空白对照组和茯苓组,每组5只,分别给予生理盐水和茯苓灌胃(0.5 mL),连续灌胃32 d。每间隔3 d计算移植瘤的体积,绘制移植瘤生长曲线。至第32天时处死裸鼠,称取瘤体重量,计算抑瘤率;同时采用免疫组化染色方法检测2组裸鼠移植瘤组织中B细胞淋巴瘤基因-2(B cell lymphoma 2,Bcl-2)、Bcl-2相关X蛋白(Bcl-2 associated X protein,Bax)、胱天蛋白酶-3(Caspase-3)、胱天蛋白酶-9(Caspase-9)及血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达。2制备含茯苓血清。将SGC-7901胃癌细胞株分为2组,空白对照组加入含生理盐水血清,茯苓组加入含茯苓血清的培养液培养。分别于处理后24 h和48 h行Western-blot法检测胃癌细胞中Bcl-2及Bax的表达。结果第32天时,空白对照组〔(2 652.17±225.01)mm3和(2.48±0.21)g〕裸鼠的移植瘤体积和质量均大于茯苓组〔(1 247.56±277.23)mm^3和(1.28±0.28)g〕,差异均具有统计学意义(P<0.050)。茯苓的抑瘤率为48.39%。免疫组化检测结果显示,茯苓组裸鼠移植瘤组织中Bcl-2〔(4.20±1.10)分和(8.00±1.20)分〕和VEGF〔(3.80±0.45)分和(7.80±1.10)分〕的表达均低于空白对照组(P<0.050),而Bax〔(7.40±1.34)分和(3.00±0.71)分〕、Caspase-3〔(6.60±1.34)分和(2.60±0.55)分〕及Caspase-9〔(7.20±1.79)分和(4.00±1.22)分〕的表达均高于空白对照组(P<0.050)。与空白对照组(1.72±0.03)比较,24 h(0.96±0.04)和48 h(0.77±0.04)时茯苓组Bcl-2的表达水平均较高(P<0.050),且茯苓组48 h时Bcl-2的表达水平高于24 h时(P<0.050);与空白对照组(0.15±0.01)比较,24 h时(0.19±0)茯苓组Bax的表达水平变化不明显(P>0.050),但48 h时(0.55±0.01)Bax的表达水平高于空白对照组和24 h茯苓组(P<0.050)。结论茯苓能够抑制裸鼠移植瘤的生长,其机理可能与线粒体凋亡通路及抑制VObjective To explore the effect of Poria cocos on xenograft tumors of gastric cancer SGC-7901 cell line in mude mice. Methods 1 After establishment of xenograft tumor of gastric cancer SGC-7901 cell line, 10 nude mice were equally divided into normal control group and Poria cocos group. The nude mice of each group were gavaged with normal saline（NS） and Poria cocos（0.5 mL） for 32 days, respectively. Tumor volume were measured to draw tumor growth curves and the tumor weight inhibitory rate was calculated with tumor weight（on the 32-day, nude mice were sacrificed to get the xenograft tumors）. The expressions of B cell lymphoma 2（Bcl-2）, Bcl-2 associated X protein（Bax）, Caspase-3, Caspase-9, and vascular endothelial growth factor（VEGF） were detected by immunohistochemical staining. 2 Preparation of drug serum containing Poria cocos. Gastric cancer SGC-7901 cell line were be divided into 2 groups： normal control group and Poria cocos group. Cells of normal control group were treated with serum containing NS, and cells of Poria cocos group were treated with drug serum containing 10% Poria cocos. After 24 hours and 48 hours,Western-blot was used to detect the expressions of Bcl-2 and Bax. Results On 32-day, the volume and weight of xenograft tumors in normal control group 〔（2 652.17±225.01） mm-3 and（2.48±0.21） g〕 were both higher than those of Poria cocos group 〔（1 247.56±277.23） mm3 and（1.28±0.28） g〕, P〈0.050. The tumor inhibitory rate in Poria cocos group was 48.39%.The results of immunohistochemical staining showed that, compared with normal control group, Poria cocos could downregulate the expressions of Bcl-2 〔（4.20±1.10）score vs.（8.00±1.20） score〕 and VEGF 〔（3.80±0.45） score vs.（7.80±1.10） score〕, while up-regulate the expressions of Bax 〔（7.40±1.34） score vs.（3.00±0.71） score〕, Caspase-3 〔（6.60±1.34） score vs.（2.60±0.55） score〕, and Caspase-9 〔（7.20±1.79） score vs.（4.00±1.22） score〕, P�

关 键 词：茯苓 SGC-7901胃癌细胞株 裸鼠移植瘤 

分 类 号：R285.5[医药卫生—中药学]