山羊STAT3基因克隆、生物信息学分析及甲基化修饰研究  

Molecular Cloning,Bioinformatics Analysis and Methylation Modification of Goat STAT3 Gene

作　　者：贾文超[1] 刘亮亮[2] 吴贤锋 赵钊艳 王珂[1] 王毛[1] 高静[1] 王立强[1] 陈宏[1] 潘传英[1] 蓝贤勇[1] 

机构地区：[1]西北农林科技大学动物科技学院/生命科学学院,陕西省农业分子生物学重点实验室,杨凌712100 [2]江苏科技大学计算机科学与工程学院,镇江212003

出　　处：《畜牧兽医学报》2016年第3期457-466,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31172184);陕西省留学人员科技活动择优资助项目(2014-14);西北农林科技大学大学生创新性实验计划(2013)

摘　　要：本研究利用RT-PCR、TA克隆和重亚硫酸盐测序(BSP)等方法,旨在克隆、分析山羊STAT3基因完整编码区,并解析该基因甲基化修饰水平及其与体重的关系。结果表明,山羊STAT3基因编码区全长2 313bp,编码770个氨基酸;与绵羊、牛、野猪、小鼠、大鼠和人的氨基酸序列一致性分别为99.7%、99.6%、99.4%、99.2%、99.4%和99.5%。山羊高体重组与低体重组之间甲基化差异研究发现,高体重组STAT3基因启动子区甲基化水平显著高于低体重组(P=0.020),提示该基因甲基化修饰对体重具有显著影响,可作为标记辅助选择(MAS)的有效表观遗传标记。这些结果为研究山羊肉用性能的遗传与表观遗传机制提供了科学资料。The aim of this study was to clone and analyze the entire coding sequence of goat STAT3 gene as well as to detect the STAT3 gene methylation modification and analyze its association with goat body weight using reverse transcription-polymerase chain reaction（RT-PCR）,TA cloning,bioinformatics and bisulfite sequencing（BSP）methods.The results showed that the entire coding sequence of goat STAT3 gene was 2 313 bp in length,and encoded 770 amino acids.The shared amino acid sequence consistency between goat（capra hircus）and sheep（Ovis aries）,bovine（Bos Taurus）,boar（Sus scrofa）,mouse（Mus musculus）,rat（Rattus norvegicus）,human（Homo sapiens）were 99.7%,99.6%,99.4%,99.2%,99.4%,99.5%,respectively.The analysis of the methylation difference in the promoter region of goat STAT3 gene between high body weight group and low body weight group demonstrated that the goats with high body weight had highermethylation level than those goats with low body weight（P=0.020）.This finding indicated that the methylation modification of STAT3 gene had significant effect on body weight in goat,which could become the epigenetic marker in marker-assisted selection（MAS）.These results would provide scientific data for studying genetic and epigenetic mechanism for meat production in goat.

关 键 词：山羊 STAT3基因 分子克隆 序列分析 DNA甲基化 

分 类 号：S827[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]