不同生长时期梅花鹿鹿茸差异蛋白质组学分析  

Comparative Proteomic Analysis in Different Growth Stages of Sika Deer Velvet Antler

作　　者：张然然[1] 刘华淼[1] 邵元臣[1] 周盼伊 苏莹[1] 王磊[1] 邢秀梅[1] 

机构地区：[1]中国农业科学院特产研究所特种经济动物分子生物学重点实验室,长春130112

出　　处：《畜牧兽医学报》2016年第3期493-501,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：特种动物遗传资源创新团队(CAAS-ASTIP-201X-ISAPS);特种动物种质资源平台

摘　　要：旨在从分子水平认识鹿茸生长机制,本研究以10、40、60与130d的梅花鹿鹿茸为试验材料,运用双向凝胶电泳(2-DE)、质谱鉴定技术与生物信息学方法对梅花鹿鹿茸生长过程中差异表达蛋白质进行研究。结果显示,有46种蛋白质差异性表达,且主要参与细胞骨架、转运过程、信号转导、细胞凋亡、骨发育、蛋白质合成、核酸代谢、免疫、能量代谢、细胞增殖、抗氧化、蛋白质折叠等生物学过程。结合鹿茸的快速生长与快速骨化的独特生长过程,对差异表达蛋白质中的骨发育相关蛋白、抗氧化蛋白、细胞凋亡相关蛋白做进一步分析,发现P4HB、SPARC、过氧化物还原酶2、过氧化物还原酶4、半乳糖凝集素1、视黄酸结合蛋白1等6种蛋白质在鹿茸快速生长与快速骨化过程中起着重要的作用,为鹿茸生长与骨化机制的进一步研究奠定基础。To understand the velvet antler development mechanism in the level molecular biology,proteome differences of the sika deer velvet antlers aged 10,40,60 and 130dwere compared using2-DE,mass spectrometry and bioinformatics.Therefore,46 proteins were altered their expressions in the sika deer velvet antlers aged 10,40,60 and 130d,which were involved in cytoskeleton,transportation,signal transduction,apoptotic process,bone development,protein synthesis,nucleotide biosynthesis,immunity,energy metabolism,cell proliferation and antioxidant activity and protein folding.We focused on the proteins involved in bone development,antioxidant activity and apoptotic process due to the special growth process with rapidly growth and ossification.Therefore,The results showed that several proteins（P4HB,SPARC,peroxiredoxin 2,peroxiredoxin 4,galectin 1and retinoic acid binding protein 1）might play vital role in the velvet antler growth and ossification process.Taken together,the result provided a basis for further research on the molecular mechanisms involved in the accelerated growth and ossification of deer velvet antler.

关 键 词：梅花鹿 鹿茸 不同生长时期 双向凝胶电泳 蛋白质组学 

分 类 号：S825.2[农业科学—畜牧学]