冷处理后水稻苗期差异蛋白的表达与分析  

Expression and Analysis of Differential Proteins of Rice Seedling after Cold Treatment

作　　者：周国华[1,2] 刘涛[2] 张雪梅[2] 张群宇[3] 李晓芬 

机构地区：[1]宜春学院生命科学与资源环境学院,江西宜春336000 [2]云南农业大学农学与生物技术学院,云南昆明650201 [3]华南农业大学生命科学学院,广东广州510642 [4]国家杂交水稻改良中心高原分中心,云南个旧661000

出　　处：《西南农业学报》2016年第3期520-524,共5页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31200219);云南省教育厅基金项目(2012Y371)

摘　　要：水稻ZH-ct为ZH11在组织培养过程中发现一株突变单株,具有较好的苗期耐冷性,通过人工气候箱低温处理,应用蛋白质双向凝胶电泳(2-DE)检测其低温处理后的差异蛋白表达,并对进行分析。结果显示,共发现差异蛋白468个,对其中重复性好,表达量较高的14个蛋白进行质谱分析,除2个没有检测到蛋白信号外,其余12个分别为光合作用、能量代谢、渗透压调节、过氧化物酶等功能蛋白,为下一步进行耐冷的分子基础及反向遗传学分析提供了基础。ZH-ct possessed the characters of good cold tolerance, which was a mutation plants from tissue culture of zhl 1 callus. Its differential protein expressions in the artificial climate chamber after low temperature treatment by 2-DE were studied. The result showed that there were 468 differential expression proteins spots signed by it＇ s relative software, in which 14 spots had a better replication and were easily to slice, which were further analyzed by mass spectra, and all of them, except 2 spots were no signs, were photosynthesis, supersession, os- motic pressure adjust, peroxide enzyme, respectively. The results would provide the foundation for rice seeding cold tolerance research and reversed genetics.

关 键 词：冷处理 水稻 苗期 差异蛋白 表达 

分 类 号：S511[农业科学—作物学]