超临界CO_2提取桉树叶挥发油工艺优选  

Optimization of Extraction Process for Volatile Oil from Eucalyptus Leaves by Supercritical CO_2 Method

作　　者：曹丽萍[1] 廖昌军[1] 臧志和[1] 王涛[1] 余中杰[1] 

机构地区：[1]成都医学院药学院实验教学中心,四川成都610083

出　　处：《成都中医药大学学报》2016年第1期22-25,共4页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：成都医学院科研项目(编号:CYZ13-028)

摘　　要：目的:研究CO_2超临界提取桉树叶挥发油的最佳工艺条件。方法:采用Box-Behnken中心组合试验设计原理,在单因素试验的基础上,采用三因素三水平的响应曲面分析法对桉树叶中桉树油提取工艺进行优化。结果:得到桉树叶挥发油提取的最佳工艺条件为:提取温度75.72℃,提取时间8 h,提取压强402.78 bar,提取率为6.83%。结论:方法简便合理、稳定,可预测性较优。Objective： To study the technological parameters of the extraction process of volatile oil in eucalyptus leaves by supercritical CO_2 technology. Methods： On the basis of one-factor-at-a-time-technique test, the Box-Behnken central-composite designed response surface with 3 factors and 3 levels was adopted. A second order quadratic equation for volatile oil from eucalyptus leaves was built. The response surface methodology（ SM） with three factors and three levels was used to optimize extraction process by supercritical CO_2 technology. Results： Optimum extraction conditions was as follow： extracted 8 h with 402. 78 bar at 75. 72℃,yield of volatile oil 6. 83 %. Conclusion： Themethod is simple,stable and feasible,and the selected process also has a good reproducibility.

关 键 词：桉树叶挥发油 CO2超临界提取 响应面分析法 工艺优化 

分 类 号：R284[医药卫生—中药学] R284.2[医药卫生—中医学]