蛇床子素抑制TNF-a表达及作用机制初探  

Inhibition of Osthole on TNF-a Production and Its Mechanism

作　　者：梁正[1] 郝文龙[2] 

机构地区：[1]成都中医药大学,四川成都611137 [2]内蒙古集宁师范学院,内蒙古集宁012000

出　　处：《成都中医药大学学报》2016年第1期26-28,113,共4页Journal of Chengdu University of Traditional Chinese Medicine

摘　　要：目的:研究蛇床子素抗类风湿关节炎作用,并初步探索其作用机制。方法:基于肿瘤坏死因子(TNF-a)在类风湿关节炎发病中的作用,设计一种抑制TNF-a产生的抗类风湿关节炎小分子药物高通量筛选新方法。结果:与DMSO对照组相比,蛇床子素(N8)能显著抑制脾脏细胞中TNF-a的产生(P<0.001),减少TNF-a基因的表达,降低p38磷酸化水平。结论:蛇床子素通过抑制p38的磷酸化,抑制TNF-a基因的表达,其具有开发成为治疗类风湿性关节炎药物的潜力。Objective： To study the inhibitory effect of Osthole on Rheumatoid arthritis and try to explain the underlying mechanism. Methods： In the light of the high expression of TNF-a in Rheumatoid arthritis,a high throughput screening system was designed to screen anti-Rheumatoid arthritis small molecule drug targeting TNF-a. Results： Compared with DMSO control group,Osthole showed a high capacity of inhibiting TNF-a production in splenocytes（ P〈0. 001）,down-regulating TNF-a mRNA expression,and decreasing the level of p38 phosphorylation. Conclusion： Osthole could inhibit the TNF-a production by down-regulating TNF-a mRNA expression through suppressing the phosphorylation of p38. Osthole is a potential drug for the treatment of Rheumatoid arthritis.

关 键 词：类风湿关节炎 筛选 蛇床子素 TNF-A P38 

分 类 号：R282.74[医药卫生—中药学] R392.7[医药卫生—中医学]