野生和栽培紫丁香种群遗传多样性的比较研究  

A Comparative Study on the Genetic Diversity of Wild and Cultivated Syringa Oblata Populations

作　　者：张恒庆[1] 陈秋月[1] 张粤[2] 唐丽丽[1] 宝超慧 郝久程 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116081 [2]中国科学院沈阳应用生态研究所,辽宁沈阳110016

出　　处：《辽宁大学学报（自然科学版）》2016年第1期56-60,共5页Journal of Liaoning University：Natural Sciences Edition

基　　金：科技部科技支撑项目(2012BAC05B05)

摘　　要：采用ISSR分子标记技术,对采自阜新丁香岭、海棠山和朝阳云蒙山、石洞沟村的4个紫丁香野生种群和大连英歌石植物园的1个栽培种群的153个个体进行了遗传多样性分析和比较.11个ISSR引物从紫丁香基因组总DNA中共扩增出82条带.分析结果表明:在物种水平上,紫丁香种群的多态性条带百分率(PPB)、Nei's基因多样性指数(h)、Shannon信息指数(I)分别为95.12%、0.360 1、0.530 4,其遗传分化系数(Gst)和基因流(Nm)分别为0.137 6和3.133 2.在种群水平上,4个野生种群HTS、SDG、DXL、YMS的多态性条带百分率分别为86.59%、73.17%、78.05%、73.17%,而栽培种群YGS的多态性条带百分率为69.51%,野生种群比栽培种群表现出较高的遗传多样性.The genetic diversity of 153 individuals that collected from four wild populations of syringa oblata in Dingxiangling and Haitangshan of Fuxin,Yunmengshan and Shidonggou of Chaoyang,and one cultivated population in Da Lian Yinggeshi botanical garden,was analyzed and compared by ISSR molecular markers technology.82 bands were generated by 11 primers.The results showed that the percentage of polymorphic band（ PPB） 、Nei＇ s diversity index（ h） and Shannon information index（ I） were respectively 95.12% 、0.3601、0.5304,their genetic differentiation coefficient（ Gst） and gene flow（ Nm） were 0.1376 and 3.1332 at the species level.And at the population level,the PPB of four wild populations were 86.59% 、73.17% 、78.05% 、and 73.17%,respectively.The PPB of cultivated population was 69.51%,the wildpopulations showed higher genetic diversity than the cultivated population.

关 键 词：ISSR 紫丁香 遗传多样性 

分 类 号：Q943.2[生物学—植物学]