miR-335在肿瘤组织中的表达及其预测靶基因的生物信息学分析  

miR-335 Expression in Tumor Tissues and Bioinformatic Analysis of Predicted Target Genes

作　　者：陈利娜[1] 滕牧洲 卢严方 郑文岭[1] 马文丽[1] 

机构地区：[1]南方医科大学基因工程研究所,广州510515

出　　处：《中国生物工程杂志》2016年第3期23-30,共8页China Biotechnology

基　　金：国家自然科学基金(39880032);广东省领军人才基金(C1030925)资助项目

摘　　要：目的:分析miR-335在多种肿瘤组织与癌旁组织中的表达,预测其靶基因并进行相关生物信息学分析,为进一步研究miR-335在肿瘤中的调控机制提供理论基础。方法:分析miR-335的保守性及在多个肿瘤组织中的表达;预测miR-335靶基因,并使用DAIVID对miR-335靶基因进行生物信息学分析。结果:miR-335序列高度保守,在肝癌、肺癌、乳腺癌、肝内胆管癌、脂肪肉瘤中表达下调(P<0.05)。预测miR-335靶基因共34个,靶基因集合功能富集于细胞迁移、凋亡、转录调控,以及蛋白质分子连接、细胞骨架组成等生物学过程和分子功能(P<0.05);主要参与了轴突向导和黏着斑信号通路、黑素瘤疾病信号通路及TGF-β信号通路(P<0.05)。结论:miR-335在多种肿瘤中表达异常,且涉及多个生物学过程和信号转导通路,与肿瘤的发生发展密切相关。Objective： Providing a theoretical basis for the further study on the mechanism of miR-335 in cancer,bioinformatics was utilized to investigate miR-335 expression in tumor tissues,and the functions of miR-335 predicted target genes were analyzed as well. Methods： Sequence alignment of miR-335 in multiple species was used to analyze its conservation. The miRNA microarray data of tumor tissues were applied for detecting miR-335 expression level between the tumor and normol tissues. Predicting miR-335 target genes by several on-line tools. DAVID was used to analyze the function and signal pathway of miR-335 target genes. Results： The sequence of miR-335 was highly conserved among different species. Compared with the normal tissues,miR-335 was low-expression in the tissues of liver cancer,lung cancer,breast cancer,intrahepatic cholangiocarcinoma and liposarcoma（ P〈0. 05）. 34 target genes were predicted,and the function of these target genes mainly enriched in cell division,cell migration,regulation of apoptosis,protein binding,regulate transcription factor activity and protein amino acid phosphorylation（ P〈0. 05）. In KEGG pathway,the predicted target genes set was involving in axon guidance signal,melanoma diseases pathway,focal adhesion and TGF-beta signaling pathway（ P〈0. 05）. Conclusion： Abnormal gene expression of miR- 335 was discovered in variety of tumor tissues. The target genes of miR-335 closely related to multiple biological processes and signal transduction pathways in cancer.

关 键 词：miR-335 肿瘤 靶基因 生物信息学 

分 类 号：Q786[生物学—分子生物学]