中药混合液对麻黄素致孕鼠肝损伤及抗氧化功能的影响  

Effects of Chinese medicine mixture on liver damage and antioxidant function of pregnant mice by injected ephedrine

作　　者：俞诗源[1,2] 彭静[1] 刘婷婷[1] 安晶晶[1] 

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070 [2]西北师范大学学报编辑部,甘肃兰州730070

出　　处：《西北师范大学学报（自然科学版）》2016年第2期95-100,共6页Journal of Northwest Normal University(Natural Science)

基　　金：甘肃省自然科学基金资助项目(1107RJZA141);兰州市社会发展项目(2013-3-72;2014-1-105)

摘　　要：为探讨中药混合液对麻黄素致损伤孕鼠肝组织的保护作用,将72例受孕小鼠随机分为对照组、麻黄素组和中药组,麻黄素组和中药组连续腹腔注射0.043g·kg-1剂量的麻黄素溶液,中药组在注射麻黄素溶液1h后灌胃0.2mL中药混合液,记录孕鼠行为的变化,比色法测定肝组织T-AOC、GST活性及MDA含量,生物显微技术观察孕鼠肝组织结构的变化.结果显示麻黄素组孕鼠饮食、饮水量减少,跳动次数增多,肝组织T-AOC、GST活性显著降低,MDA含量明显升高,肝组织出现不同程度的损伤,肝血窦扩张,内皮细胞脱落,肝小叶界限紊乱,肝细胞空泡化;中药组孕鼠饮食、饮水量增多,跳动次数减少,肝组织T-AOC、GST活性显著升高,MDA含量显著降低,肝组织结构损伤明显减轻,肝小叶界限清晰,内皮细胞脱落减少,肝细胞结构基本正常.表明麻黄素抑制细胞的抗氧化酶活性,对肝组织结构有明显的损伤作用,而中药混合液可显著提高组织细胞的抗氧化能力,改善细胞活力,对麻黄素致损伤孕鼠肝组织有一定的保护作用.To explore the effects of Chinese medicine mixture on ephedrine-induced liver damage in the pregnant mice,the 72 pregnant mice are randomly divided into control group,ephedrine group and Chinese medicine group.Ephedrine group and Chinese medicine group are injected with 0.043g·kg-1doses of ephedrine.Chinese medicine group is intragastric with 0.2 mL Chinese medicine mixture after injected ephedrine 1h.The changes of the behaviour of pregnant mice are recorded.The changes of the activity of T-AOC,GST are detected as well as the content of MDA by colorimetry,and the changes of liver tissue structure are observed by microscopy.Result shows that the eating and drinking reduce,jumping number increases,the activity of T-AOC and GST decrease significantly,the content of MDA increases in the liver in ephedrine group,and the tissue structure has different degrees damaged,for example,hepatic sinusoid expansion,endotheliocyte off,hepatic lobules boundaries disorder and liver cell vacuoles.In Chinese medicine group,the eating and drinking increase,jumping number decreases,the activity of T-AOC and GST are higher,and the content of MDA is lower.The damage of the tissue structure reduces significantly.Liver lobule boundary is clear.Endotheliocyte off is reduced significantly,and liver cell structure is normal.Ephedrine can inhibit the activity of antioxidant enzymes of the cell and has obvious damage in the liver tissue structure.The Chinese medicine mixture can significantly improve the antioxidant activity of the cell and helps to protect ephedrine-induced liver damage of the pregnant mice.

关 键 词：中药混合液 麻黄素 孕鼠 肝 组织结构 抗氧化酶 

分 类 号：R338.21[医药卫生—人体生理学]