牛膝多糖对过氧化氢损伤人脐静脉内皮细胞的保护作用  

Protective effect of Achyranthes bidentata polysaccharideson on human umbilical vein endothelial cell injured by hydrogen peroxide

作　　者：王菊平[1] 宋献美 钱丽丽 

机构地区：[1]郑州市儿童医院,河南郑州450003 [2]河南医学高等专科学校,河南郑州451191

出　　处：《中医药临床杂志》2016年第2期245-247,共3页Clinical Journal of Traditional Chinese Medicine

基　　金：河南省高等学校重点科研项目(NO.15B310004)

摘　　要：目的:观察牛膝多糖对过氧化氢(H2O2)诱导人脐静脉内皮细胞(HUVEC)损伤的保护作用。方法:体外培养HUVEC,分为空白对照组、模型对照组、牛膝多糖低、中、高浓度组(浓度为5%、10%、15%),显微镜观察细胞形态;微板法检测细胞上清液中乳酸脱氢酶(LDH)的活性及一氧化氮(NO)的含量,ELISA法检测内皮素-1(ET-1)、组织型纤溶酶原激活物(t PA)、纤溶酶原激活物抑制剂(PAI-1)的含量。结果:与模型对照组比较,牛膝多糖中、高浓度组细胞形态改善,LDH活性显著减低、ET-1和PAI-1含量显著减少,NO和t PA显著升高。结论:牛膝多糖对H2O2诱导的HUVEC损伤具有显著地保护作用。Objective： To observe the protective effect of Achyranthes bidentata polysaccharideson the injury of human umbilical veinendothelial cell（HUVEC） injured by hydrogen peroxide. Methods： The HUVEC were cultured vitro and divided into five groups as follows： control group, model group, low-dosage of Achyranthes bidentata polysaccharides, mid-dosage of Achyranthes bidentata polysaccharides group and high-dosage of Achyranthes bidentata polysaccharides. Cell morphology was observed under inverted phase contrast microscope. The contents of lactate dehydrogenase（LDH）, nitric oxide（NO） were measured by the micro-plate and the method of enzyme-linked immunosorbent assay（ELISA） was used to determine endothelin-1（ET-1）, tissue plasminogen activator（t PA） and plasminogen activator inhibitor-1（PAI-1）. Results： Compared with the model group, the mid-dosage of Achyranthes bidentata polysaccharides and high-dosage of Achyranthes bidentata polysaccharides significantly improved HUVECs morphology, decreased the contents of LDH, ET-1, PAI-1 and increased the contents of NO and t PA（P〈0.05 or P〈0.01）. Conclusion： Achyranthes bidentata polysaccharideshas significant protective effect on HUVEC injured by H2O2.

关 键 词：牛膝多糖 人脐静脉内皮细胞 过氧化氢 保护 

分 类 号：R965[医药卫生—药理学]