HPLC法测定癃闭舒片中补骨脂素和异补骨脂素  

Determination of psoralen and isopsoralen in Longbishu Pills by HPLC

作　　者：王志成 时圣明[2] 史琳[3] 毛雨葳 

机构地区：[1]大连市食品药品检验所,辽宁大连116021 [2]天津药物研究院,天津300193 [3]沈阳农业大学,辽宁沈阳110866

出　　处：《药物评价研究》2016年第1期97-100,共4页Drug Evaluation Research

基　　金：辽宁省自然科学基金优秀人才培养项目(2015020698)

摘　　要：目的建立癃闭舒片中补骨脂素和异补骨脂素的定量方法。方法对国内3个企业生产的6个批次的癃闭舒片,采用HPLC法检测,Agilent TC-C18（2）色谱柱（250 mm×4.6 mm,5μm）,流动相是甲醇-水（43？57）,体积流量1.0 mL/min,检测波长为246 nm,柱温30℃。结果补骨脂素回归方程为：Y=84.44X＋9.994,r=0.999 9（n=12）。异补骨脂素回归方程为：Y=82.434X＋8.8952,r=0.999 9（n=12）。补骨脂素和异补骨脂素的线性范围均在4.04-151.5μg。结论本方法操作简便、准确、专属性强、稳定,可用于癃闭舒片中补骨脂素和异补骨脂素的含量测定。Objective To establish a method for the content determination of psoralen and isopsoralen in Longbishu Pills（LBSP）. Methods HPLC was performed on the Agilent TC-C18（2） column（250 mm×4.6 mm, 5 μm） with the mobile phase of Me OH-H2O（43：57）, the flow rate was 0.8 mL/min, the detection wavelength 246 nm, and the column temperature was 30℃. Results The element regression equation of psoralen was Y=84.44 X ＋ 9.994, r=0.999 9（n=12）. The element regression equation of isopsoralen was Y=82.434X＋8.895 2, r=0.999 9（n=12）. The concentrations of psoralen and isopsoralen were within the range of 4.04—151.5 μg. Conclusion The method is simple, reliable, and specific, with good reproducibility, and could be used to determine the content of psoralen and isopsoralen in Longbishu Pills.

关 键 词：高效液相法 癃闭舒片 补骨脂素 异补骨脂素 含量测定 

分 类 号：R917[医药卫生—药物分析学]