冬虫夏草人工培殖过程中寄主昆虫粉拟青霉病原菌的分子生物学研究  

Progress on molecular biology of Isaria farinosa,pathogen of host of Ophiocordyceps sinensis during the artificial culture

作　　者：刘飞[1,2,3] 伍晓丽[1,2,3] 刘莹[4] 陈大霞[1,2,3] 张德利[1,2,3] 杨大坚[1,2,3] 

机构地区：[1]重庆市中药研究院,重庆400065 [2]重庆市中药资源学重点实验室,重庆400065 [3]中国中医科学院中药资源中心重庆分中心,重庆400065 [4]中国农业大学农学与生物技术学院,北京100193

出　　处：《中国中药杂志》2016年第3期403-409,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30901964;81072997;81173479);重庆市自然科学基金项目(CSTC 2010BB5344);重庆市基本科研业务费计划项目(JA-15-16)

摘　　要：虫草棒束孢Isaria farinosa是冬虫夏草人工培殖过程中寄主昆虫粉拟青霉病的病原菌。该文通过统计分析国内外相关文献和NCBI网站上登录的相关序列和基因信息,分析虫草棒束孢的分子生物学研究进展。结果发现不同国家发表的文献不同,登录的相关序列或基因的种类和数量存在差异。我国发表的文献和登录的序列或基因总量都最多,美国登录的功能基因总量最多。相关文献主要涉及粉拟青霉病在生物防治方面的应用。而与分子生物学相关的研究主要集中在该菌的系统分类学上;近年来开始了与虫草棒束孢作为生防菌相关的蛋白酶基因和几丁质酶基因等的研究。随着冬虫夏草在健康领域影响力的增强,以及虫草棒束孢全基因组序列的测序完成和药理活性的不断揭示,对虫草棒束孢分子水平的研究也必将越来越广泛和深入。Isaria farinosa is the pathogen of the host of Ophiocordyceps sinensis. The present research has analyzed the progress on the molecular biology according to the bibliometrics,the sequences（ including the gene sequences） of I. farinosa in the NCBI. The results indicated that different country had published different number of the papers,and had landed different kinds and different number of the sequences（ including the gene sequences）. China had published the most number of the papers,and had landed the most number of the sequences（ including the gene sequences）. America had landed the most numbers of the function genes. The main content about the pathogen study was focus on the biological controlling. The main content about the molecular study concentrated on the phylogenies classification. In recent years some protease genes and chitinase genes had been researched. With the increase of the effect on the healthy of O. sinensis,and the whole sequence and more and more pharmacological activities of I. farinosa being made known to the public,the study on the molecular biology of the I. farinosa would be deeper and wider.

关 键 词：虫草棒束孢 冬虫夏草菌寄主昆虫 冬虫夏草菌 分子生物学 

分 类 号：S435.673[农业科学—农业昆虫与害虫防治]