响应面法优化泽泻中23-乙酰泽泻醇B闪式提取工艺  

Optimization of flash-type extraction technology of alisol B 23-acetate from Alismatis Rhizoma by response surface methodology

作　　者：韦迎春[1,2] 闫明[1,2] 杨晶[1,2] 刘俊超[1,2] 殷洪梅[1,2] 吴云[1,2] 孙永城[1,2] 萧伟[1,2] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222047 [2]中药制药过程新技术国家重点实验室,江苏连云港222047

出　　处：《中国中药杂志》2016年第3期438-442,共5页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2013ZX09402203)

摘　　要：采用响应面分析法优化得到23-乙酰泽泻醇B闪式提取的最佳工艺。采用单因素考察法,以23-乙酰泽泻醇B提取率为指标,考察乙醇浓度、液料比、提取次数、提取时间对23-乙酰泽泻醇B提取率的影响,并结合Box-Behnken试验设计和响应面分析方法对闪式提取泽泻的工艺参数进行优化,得到23-乙酰泽泻醇B闪式提取最佳工艺为80%乙醇、液料比12∶1、提取4次、提取时间114 s/次。通过响应面法优化得到的23-乙酰泽泻醇B闪式提取工艺,稳定、省时、高效,且具有可室温提取、无成分破坏的优点,可用于实际大生产。Response surface methodology was used to optimize and obtain the optimal flash-type extraction technology of alisol B 23-acetate from Alismatis Rhizoma. With the extraction rate of alisol B 23-acetate as an indicator,single-factor test was used to investigate the effect of ethanol volume fraction,liquid-solid ratio,extraction times and extracting time on the extraction rate of alisol B 23-acetate. The results were combined with Box-Benhnken design and response surface analysis to optimize the technology parameters for extraction process of Alismatis Rhizoma and obtain the optimal flash-type extraction technology under the following conditions： ethanol volume fraction 80%,liquid-solid ratio 12∶ 1,extraction 4 times,114 s / time. Flash-type extraction technology of alisol B 23-acetate by response surface methodology is stable,time-saving,efficient,and with the advantages of room temperature extraction and no component damage,so it can be used for massive production.

关 键 词：23-乙酰泽泻醇B 闪式提取 响应面法 

分 类 号：R284.2[医药卫生—中药学]