白藜芦醇与紫杉醇联合用药对人喉癌Hep-2细胞凋亡机制的研究  

Apoptosis mechanism of taxol combined with resveratrol on human laryngeal carcinoma Hep-2 cells

作　　者：卢晨欣 孙警辉[1] 伍春莲[1,2] 

机构地区：[1]西南野生动植物资源保护教育部重点实验室西华师范大学,四川南充637009 [2]西南大学三峡库区生态环境与生物资源省部共建国家重点实验室,重庆400715

出　　处：《中国中药杂志》2016年第3期476-483,共8页China Journal of Chinese Materia Medica

基　　金：四川省教育厅重大培育项目(13CZ0029);三峡库区生态环境与生物资源省部共建重点实验室开放课题(SKL-2011-05);中国博士后基金项目(2013M540391)

摘　　要：喉癌是呼吸道肿瘤中最常见的恶性肿瘤之一,在呼吸道肿瘤中高居第二位。白藜芦醇(resveratrol,Res)是一种多酚类物质,可通过抑制细胞内核苷酸还原酶抑制肝癌细胞、胃癌细胞、胰腺癌细胞等肿瘤细胞生长。紫杉醇(taxol,Tax)是红豆杉属植物中一种次生代谢物,可抑制细胞内微管的解聚,对乳腺癌、宫颈癌、卵巢癌等肿瘤有良好的抗肿瘤活性。目前有关白藜芦醇联合紫杉醇用药针对人喉癌细胞株Hep-2的作用及其相关分子机制研究还鲜见报道。用白藜芦醇(Res)、紫杉醇(Tax)处理人喉癌细胞株Hep-2,采用CCK-8法检测2种药物对Hep-2细胞增殖的影响,AO/PI染色和JC-1检测Hep-2细胞凋亡,实时定量PCR检测Bax,Bcl-2,PARP,TRIB3,XIAP基因的表达,荧光定量法检测caspase-3,caspase-8活性。结果表明,与Tax,Res单独用药相比,联合组显著增强对Hep-2细胞的抑制作用,且明显减少Tax的用药剂量,增加Bax,PARP,TRIB3的表达,降低Bcl-2,XIAP的表达,促进caspase-3,caspase-8活性。这表明Res,Tax以及其联合药物通过线粒体途径诱导喉癌细胞Hep-2的细胞凋亡。这为进一步研究Res,Tax联合用药治疗喉癌奠定理论基础,且扩大Res,Tax联合用药范围。Laryngeal cancer is one of the most common malignant tumors in the respiratory tumors,and its incidence ranks second highest in the respiratory tumors. Resveratrol（ Res） is a kind of polyphenols,which can inhibit nucleotides can inhibit the growth of liver cancer cells,gastric cancer cells,pancreatic cells and other tumor cells by inhibiting ribonucleotide reductase in the cells. Taxol（ Tax） is a kind of secondary metabolites of Taxus chinensis,which has anti-tumor activity for breast cancer,cervical cancer,ovarian cancer and other tumors by inhibiting cellular microtubule depolymerization. But at present the effects of resveratrol combined with taxol on human laryngeal carcinoma cell strain Hep-2 and their underlying molecular mechanisms are rarely reported. After human laryngeal cancer cell Hep-2 cells were processed with resveratrol（ Res） and taxol（ Tax）,CCK-8 assay was used to evaluate the effect of these two herbs on the proliferation of cancer cells; AO / PI staining and JC-1 were used to detect Hep-1 cells apoptosis; the expression of Bax,Bcl-2,PARP,TRIB3,and XIAP genes was detected by real time quantitative PCR; the activity of caspase-3 and caspase-8 was determined with quantitative fluorescence method. The experimental results showed that compared with Tax,Res medication alone,joint group significantly enhanced inhibition of Hep-2 cells activity,decreased the dosage of Tax,increased the expression of Bax and PARP,TRIB3,reduced the expression of the Bcl-2 and XIAP,and promoted the activity of caspase-3 and caspase-8. The test results showed that compared with the single medication,combined group could significantly increase the inhibitory effect on Hep-2 cells,significantly reduce Tax dosage,increase expressions of Bax,PARP,TRIB3,reduce expressions of Bcl-2,XIAP,and promote activity of caspase-3,caspase-8. This indicated apoptosis of human laryngeal carcinoma cell strain Hep-2 may be induced with Res,Tax,and the combination of these two herbs by mitochondria pathway. It provides valuable

关 键 词：白藜芦醇 紫杉醇 喉癌细胞株HEP-2 细胞凋亡 

分 类 号：R285[医药卫生—中药学]