玄参提取物化学成分及其大鼠血浆移行成分的LC-LTQ-Orbitrap分析  

LC-LTQ-Orbitrap analysis on chemical constituents in Scrophulariae Radix extract and their metabolites in rat plasma

作　　者：徐多多[1] 庞欢欢[1] 江美芳[1] 简伟杰[1] 王勤辉[1] 孙璐[1] 董姊怡 黄建梅[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国中药杂志》2016年第3期521-527,共7页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2011ZX09201-201-22)

摘　　要：运用HPLC-LTQ-Orbitrap技术,分析玄参提取物体外化学成分及给药后大鼠血中移行成分。采用Venusil MP C18色谱柱梯度洗脱进行分离,负离子模式下采集谱图。通过与文献及部分对照品比对,综合分析总离子流色谱图、提取离子流图以及质谱图等信息,对玄参提取物的化学成分及其血中移行成分进行定性研究。共推断出玄参提取物中37个化学成分,其中12个环烯醚萜苷类成分,20个苯丙素类成分,5个未知成分;确定哈巴苷、哈巴俄苷和安格洛苷C均以原型入血,并推测出其他2个原型成分及2个代谢产物。该研究能够为阐明玄参的药效物质基础提供一定依据,也对进一步研究玄参有重要意义。Chemical constituents in extract of Scrophulariae Radix and their metabolites in rat plasma after oral administration were identified by HPLC-LTQ-Orbitrap. Samples were separated by a Venusil MP C18 column using a binary gradient elution. The information on the total ion chromatogram,the extraction chromatogram and the mass spectrogram in a negative mode were synthetically analyzed by comparing the retention time,MS and MS / MS spectra with literature data and some of reference standards to conduct a qualitative study on constituents of Radix Scrophulariae extract in vivo and in vitro. Totally 37 compounds from Scrophularia ningpoensis extract were detected including 12 iridoid glycosides,20 phenylpropanoids and 5 unknown compounds. In vivo,harpagide,harpagoside and angoroside C were confirmed to enter into the blood in prototype forms. Besides,another 2 prototype compounds and 2 metabolites were detected in rat plasma after oral administration of S. ningpoensis extract. The results are beneficial for the determination of bioactive substances of S. ningpoensis and significant for further studies on S. ningpoensis.

关 键 词：玄参 LC-LTQ-Orbitrap 化学成分 入血成分 

分 类 号：R285.5[医药卫生—中药学] R284.1[医药卫生—中医学]