绵羊脂肪型脂肪酸结合蛋白基因(A-FABP)的变异研究  

Variations on ovine adipocyte fatty-acid binding protein(A-FABP)gene

作　　者：徐蕾[1] 闫伟[1] 胡江[1] 刘秀[1] 李少斌[1] 王继卿[1] 罗玉柱[1] 

机构地区：[1]甘肃农业大学动物科学技术学院甘肃省草食动物生物技术重点实验室甘肃省牛羊基因改良工程实验室,甘肃兰州730070

出　　处：《甘肃农业大学学报》2016年第1期29-34,共6页Journal of Gansu Agricultural University

基　　金：甘肃农业大学盛彤笙科技创新基金(GSAU-STS-1421);甘肃省创新研究群体计划(1210RJIA005);国家科技支撑计划(2012BAD13B05);国家国际科技合作专项(2011DFG33310);甘肃省国际科技合作专项(1304WCGA178)

摘　　要：【目的】明确绵羊A-FABP(adipocyte fatty-acid binding protein,A-FABP)基因的变异和单倍型特征.【方法】基于Ensemble数据库中绵羊A-FABP基因全序列,对7个绵羊品种共20个样本的A-FABP基因全序列进行测序分析.【结果】PCR扩增获得绵羊A-FABP基因全长6474bp,A、T、G、C 4种碱基的比例分别为31.48%、33.02%、17.21%和18.29%,A+T平均含量为64.50%,G+C平均含量为35.50%;共发现48处单碱基变异位点和2处微卫星位点M1((TG)n)和M2((TA)n),单一变异位点、2核苷酸变异位点和3核苷酸变异位点比例分别为28.85%、40.4%和0.02%;共发现转换、颠换、插入和缺失4种变异类型,其占变异位点总数的比例分别为45.83%、31.25%、2.08%及20.83%;共发现19种单倍型,单倍型多样度为0.995.【结论】A-FABP基因19个单倍型序列的NJ树分化为2个聚类簇,表明A-FABP基因单倍型最初由2个主要单倍型衍化形成.【Objective】The paper is aimed at revealing genetic variation and the characteristics of haplotype in sheep.【Method】Based on the complete sequence of ovine A-FABP gene in Ensemble database,the A-FABP gene was sequenced and analyzed in 20 samples collected from 7breeds.【Result】The sequence（6 474bp）of the A-FABP gene was obtained by the PCR process.The average percent of four bases（A,T,G and C）was 31.48%,33.02%,17.21% and 18.29%,respectively,the average content of A＋T and G＋C is 64.50% and 35.50%,respectively.Forty eight variation sites and two microsatellite loci for M1（（TG）n）and M2（（TA）n）were found,the percentage of single variation site,two variation sites and three variation sites was 28.85%,40.4% and 0.02%,respectively.4variation types（transition,transversion,insertion and deletion）were observed,and their ratio was 45.83%,31.25%,2.08%and 20.83%,respective-ly.19 haplotypes were found and the haplotype diversity was 0.995.【Conclusion】The NJ tree constructed by nineteen haplotypes developed two main clusters,which indicated that 19 haplotypes initially derived from two main haplotypes.

关 键 词：绵羊 A-FABP基因 变异 

分 类 号：S826[农业科学—畜牧学]