海岛棉Sus活性变化特征及时空表达模式与纤维比强度的关系  

Activity Changes and Expression Profiles of Sus with Relation to Fiber Strength in Island Cotton(Gossypium barbadense L.)

作　　者：贾春平[1] 耿洪伟[1] 倪志勇[1] 朱亚夫[1] 黄启秀[1] 曲延英[1] 陈全家[1] 

机构地区：[1]新疆农业大学农学院/农业技术重点实验室,乌鲁木齐830052

出　　处：《西北植物学报》2016年第2期249-256,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：新疆维吾尔自治区高技术研究发展计划(201411103)

摘　　要：以海岛棉(Gossypium barbadense L.)品种‘C6015’和‘新海29’(高比强度组)以及‘巴1248’和‘比马1’(低比强度组)为实验材料,利用果糖和UDPG比色法以及qRT-PCR方法,对2个实验组不同纤维发育时期蔗糖合成酶(EC 2.4.1.13,Sus)活性变化特征及其基因家族时空表达模式进行测定,并分析与纤维比强度的关系,探讨海岛棉纤维比强度差异形成的主要生理与分子机理。结果显示:(1)品种‘C6015’和‘新海29’的平均纤维比强度分别为47.5和44.7cN·tex-1,‘巴1248’和‘比马1’分别为31.2和32.6cN·tex-1,两实验组平均纤维比强度差异极显著。(2)纤维发育过程中4个海岛棉品种的Sus活性变化特征均呈单峰曲线,且低比强度组的峰值出现较早,但高比强度组的峰值以及后期活性极显著高于低比强度组。(3)海岛棉纤维发育过程中高表达的Sus基因有Sus1A、Sus1 D、Sus3A、Sus3 D、Sus6A、Sus6 D、Sus8 D,但各基因成员在纤维发育过程中具有表达特异性;其中两实验组的Sus3A基因都是在纤维次生壁加厚初期(花后20d)开始大量表达且达最大值后下降,说明Sus3A基因在纤维次生壁加厚初期起作用;Sus1A、Sus1 D基因在高比强度实验组的纤维次生壁加厚后期和末期(花后30d)相对表达量较高并有明显上升现象,而同期在低比强度实验组中相对表达量很低且无上升现象,说明Sus1A、Sus1 D基因作用于纤维次生壁加厚后期和末期。(4)两实验组的Sus活性水平及其基因家族各成员相对表达量高低与纤维次生壁加厚后期维持高活性时间的长短存在明显差异,表现为高比强度组>低比强度组;且两组Sus活性高低差异与Sus3A、Sus1A、Sus1 D基因的表达差异同步。研究表明,海岛棉Sus3A、Sus1A、Sus1 D基因的表达差异与纤维比强度的形成有关,可能是影响纤维比强度的关键基因。In this study,we used island cotton（Gossypium barbadense L.）variety as experimental materials,which are‘C6015’and ‘Xinhai 29’,（high fiber strength experimental group）;‘Ba 1248’and ‘Bima1’,（low fiber strength experimental group）.Activity changes and expression profiles of sucrose synthase（EC 2.4.1.13,Sus）during different development periods were measured by fructose and UDPG colorimet-ric with qRT-PCR methods in two experimental groups,which differences of fiber strength are obvious.Analysis the relationship between activity changes,expression profiles of Sus and fiber strength.In addition,we discussed the main physiological and molecular mechanisms,which lead to form differences of fiber strength in island cotton.The results showed that：（1）the average strength of‘C6015’and ‘Xinhai 29’：47.5cN·tex-1 and 44.7cN·tex-1,respectively;the average strength of‘Ba 1248’and ‘Bima 1’：31.2cN·tex-1 and 32.6cN·tex-1,respectively.The difference was extremely significant about the average strength in two experimental groups.（2）Activity change features of Sus were all single peak curve appear in 4island cotton varieties,as well as peak appear more early in low fiber strength experimental group.However,peak and later activity of Sus in high fiber strength experimental group were higher and reached to very significant as compared with the low fiber strength experimental group.（3）High expression of Sus genes were Sus1 A,Sus1D,Sus3 A,Sus3D,Sus6 A,Sus6D and Sus8 D,but each member of Sus genes had specific expression during fiber development in island cotton.Expression of Sus3 A gene start increased to maximum then droped at initial stage of secondary wall thickening（20days post anthesis）in two experimental groups.Showed that it emphasized on the role of this period;expression of Sus1 Aand Sus1Dgenes happened increased phenomenon at late and final period of secondary wall thickening（30-35 days post anthesis）in high fiber strength experimental group.However,expr

关 键 词：海岛棉 蔗糖合成酶活性 纤维比强度 表达模式 

分 类 号：Q789[生物学—分子生物学]