中国特有濒危植物翅果油树的SSR引物开发及特性（英文）  

Development and Characterization of Microsatellite Markers in the Elaeagnus mollis Diels

作　　者：叶占洋 王兆山[1,2,3] 李云晓[1,2] 饶国栋[1,2,3] 张建国[1,2,3] 

机构地区：[1]中国林业科学研究院林业研究所林木遗传育种国家重点实验室,北京100091 [2]中国林业科学研究院林业研究所国家林业局林木培育重点实验室,北京100091 [3]南京林业大学南方现代林业协同创新中心,南京210037

出　　处：《西北植物学报》2016年第2期274-279,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：中央级公益性科研院所基本科研业务费专项资金(CAFYBB2016ZX003-1)

摘　　要：翅果油树是中国特有的濒危植物,利用磁珠富集法开发适用于翅果油树的SSR引物,并在2个翅果油树群体进行多态性验证。结果表明,筛选的20对引物中有19对是多态且选择中性的SSR引物,其中引物CGY19表现单态,仅有一个位点显示Hardy-Weinberg平衡的偏离（P〈0.01）,在2个群体中均未检测到连锁不平衡现象;每个位点的等位基因数为1～9个,平均为4.225个,观测杂合度和期望杂合度的变化范围分别为0～0.900和0～0.847。利用开发的19对多态性SSR引物对2个翅果油树自然群体的遗传多样性水平分析结果表明,其平均遗传多样性水平为97.37%,说明翅果油树濒危的原因并不是遗传多样性水平丧失;遗传分化系数FST为0.033 1,表明遗传变异主要存在于种群内,这可能是由于种群之间的基因流（Nm=32.081 8）较大的结果,这也是2个翅果油树种群遗传相似度高的原因。这些特异性引物的开发和遗传多样性的研究将对翅果油树的交配系统、遗传结构的研究起到重要的作用,并为翅果油树种质资源的取样策略、保护和利用提供有效的信息。Elaeagnus mollis Diels is an endemic and endangered tree in China.Twenty primer pairs were selected by the combined biotin capture and identified in two populations of E.mollis.Nineteen pairs showed highly polymorphic,and were selectively neutral and the locus CGY19 was monomorphic.One locus manifested significant deviation from Hardy-Weinberg Equilibrium（P〈0.01）,which was probably caused by the influence of selection.No significant linkage disequilibrium was detected among pairs of loci in each pair.With a mean of 4.225,the number of alleles per locus ranged from 1to 9.The observed and expected heterozygosity（HOand HE）per locus ranged from 0to 0.900 and from 0to 0.847,respectively.The genetic diversity levels of 2natural populations of E.mollis were estimated by these 19 pairs of SSR markersobtained in this study.It showed that the mean genetic diversity was 97.37%,indicating that the loss of genetic diversity was not the cause of the endangerment of E.mollis.The significant fragmentation（FST=0.0331）was tested between the two populations and it provided the evidence that the genetic variation mainly existed in the populations.Furthermore,due to the frequent gene flow between populations（Nm=32.081 8）,it also verified the Shannon’s information index（I=genetic similarity）was very high in each population.On a long view,the new set of loci and the information of genetic diversity will be applied in the mating system and population structure of this species and provide valuable information for its sampling strategy,conservation and utilization.

关 键 词：翅果油树 微卫星引物 遗传结构 保护和利用 

分 类 号：Q346.5[生物学—遗传学] Q789