油松根际外生菌根真菌龟裂秃马勃的分离鉴定及培养  

Isolation,Identification and Cultivation of Ectomycorrhizal Fungus Handkea utriformis in the Rhizosphere of Pinus tabulaeformis

作　　者：张好强[1] 余红霞[1] 唐明[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100

出　　处：《西北植物学报》2016年第2期419-425,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：林业公益性行业科研专项(201404217);国家自然科学基金(31170567);西北农林科技大学博士科研启动基金(Z109021503)

摘　　要：该研究采用组织分离法对黄土高原子午岭林区连家砭林场油松根际外生菌根真菌进行了分离,并采用ITS序列对分离的真菌进行鉴定,研究了菌根真菌的培养条件,为菌剂的生产奠定基础。结果表明:子午岭林区油松根际分离培养出的真菌为龟裂秃马勃(Handkea utriformis),能够与油松形成外生菌根。采用MMN培养基为基础培养基,纯培养条件下,龟裂秃马勃生长的最适pH为6.0,最适温度为25℃。以蔗糖为唯一碳源的培养基中龟裂秃马勃生长速度最快,其次是麦芽糖和葡萄糖,生长最慢的为乳糖,在以几丁质和碳酸氢钠为碳源的培养基上不生长。有机氮源中,对脯氨酸为唯一氮源的利用能力最好,其次是酪蛋白,尿素最差;无机氮源中,以磷酸氢二铵为唯一氮源时生长最好,其次是硝酸钙,最差为酒石酸铵。该研究首次报道了黄土高原子午岭林区外生菌根真菌龟裂秃马勃,并初步确定了其最佳培养条件。The ectomycorrhizal fungus from Ziwuling forest region on the Loess Plateau was isolated by tissue isolation technique,identified based on ITS sequence,and the cultivation condition in vitro was further investigated to establish the basis of inoculum production.Result showed the isolated fungus from Ziwuling forest region was Handkea utriformis,which could form ectomycorrhiza with Pinus tabulaeformis.Using MMN medium,the optimum pH was 6.0and temperature was 25 ℃.Growth rate of H.utriformis on MMN medium with sucrose was the fastest,followed by maltose and glucose,and the slowest growth rate was for lactose.This fungus did not grow on medium using chitin and sodium bicarbonate as carbon source.For organic nitrogen source,H.utriformis grow fastest on MMN medium with proline,followed by casein,and slowest with urea.For inorganic nitrogen source,H.utriformis grow fastest with diammonium hydrogen phosphate,followed by calcium nitrate,and slowest with ammonium molybdate.For the first time,this study reported the ectomycorrhizal fungus H.utriformis in the Ziwuling region on the Loess Plateau,and its optimum growth condition in vitro was preliminary confirmed.

关 键 词：龟裂秃马勃 外生菌根真菌 黄土高原子午岭林区 最佳培养条件 

分 类 号：Q93-331[生物学—微生物学]