钙激活氯通道密度调节Anoctamin 1电流作用的研究  

Effect of Ca^(2+)-activated Chloride Channel Density on Gating Properties of Anoctamin 1

作　　者：马可[1] 王慧[1] 王清华[1] 雒舒雅 肖庆桓 

机构地区：[1]中国医科大学药学院离子通道药理研究室,沈阳110122

出　　处：《中国医科大学学报》2016年第4期298-300,共3页Journal of China Medical University

基　　金：国家自然科学基金(31371145;81572613)

摘　　要：目的研究钙激活氯通道(CaCCs)密度对CaCCs蛋白—Anoctamin 1(Ano1)电流特性的调节作用,探讨高表达Ano1促进肿瘤发生的机制。方法瞬时转染Ano1质粒到HEK293细胞,高密度CaCCs通过表达Ano1 24 h获得,低密度CaCCs通过表达Ano16 h获得。膜片钳方法检测钙离子激活的Ano1的全细胞电流。激活电流曲线以指数曲线拟合。结果 Ano1质粒表达6 h的电流密度显著低于表达24 h的电流密度(P<0.05)。在低CaCCs密度时,Ano1的激活电流曲线最适于用单指数拟合,τslow为(292.71±38.11)ms。在高CaCCs密度时,Ano1的激活电流曲线最适于用两指数拟合,τfast为(47.78±4.58)ms;τslow为(385.74±71.44)ms。高CaCCs密度下的Ano1电流比低CaCCs密度下的Ano1电流多了一个快速激活成分(τfast),而高CaCCs密度与低CaCCs密度比较Ano1的τslow差异没有统计学意义(P>0.05)。结论 CaCCs的密度可调节Ano1激活的动力学变化;高表达Ano1可促进CaCCs的激活。Objective To investigate the effect of channel density on the gating properties of Anoctamin 1 （Anol, TMEM16A） Ca2＋-activated chlo- ride channel. Methods Anol expression plasmids were transiently transfected into HEK293 cells. High density and low density of Anol was ob- tained after expressing the protein for 24 h and 6 h, respectively. Electrophysiological recordings were performed in the whole-cell patch clamp con- figuration. The activation kinetics of current traces was fitted by exponentials. Results The current density was significantly higher in cells express- ing Anol for 24 h than those expressing Anol for 6 h （P 〈 0.05 ）. The activation of Anol current in cells with low density was well fit by a single expo- nential with Tslow of 292.71±38.11 ms. The activation of Anol current in cells with high density was well fit by two exponentials with Tfast of 47.78±4.58 ms and Tslow of 385.74±71.44 ms. ANO1 current in cells with high density has a rapid active component （Tfast） more than low density. There was no significantly different of the Tslow between cells with high density and low density of Ano 1 （P 〉 0.05 ）. Conclusion Our findings suggested that chan- nel density regulates the gating of Anol. High channel density promotes activation of Anol.

关 键 词：Anoctamin 1 钙激活氯通道 通道密度 激活动力学 肿瘤 

分 类 号：R966[医药卫生—药理学]