肺癌中异常增高的RAD21的临床意义及潜在功能预测  

Clinical Significance and Functional Prediction of Up-regulated RAD21 in Lung Cancer

作　　者：谷学静[1] 吴月明[2] 高静[3] 

机构地区：[1]辽宁医学院人体解剖学教研室,辽宁锦州121001 [2]辽宁医学院附属第三医院肿瘤科,辽宁锦州121001 [3]辽宁医学院附属第一医院超声科,辽宁锦州121001

出　　处：《中国医科大学学报》2016年第4期328-332,共5页Journal of China Medical University

基　　金：辽宁省自然科学基金(2015020366);辽宁省博士科研启动基金(201501102)

摘　　要：目的检测肺癌组织中RAD21(S.Pombe RAD21同系物)的表达水平,探讨其潜在临床意义及功能。方法采用Western blot和免疫组织化学方法检测肺癌组织中RAD21的表达水平;通过Oncomine(肿瘤微阵列数据库)分析RAD21 mRNA在肺癌中的表达情况;应用Kaplan-Meier分析RAD21的表达水平与肺癌患者预后的关系;采用基因集富集分析方法预测RAD21在肺癌中的功能。结果与癌旁组织相比,肺癌组织中RAD21蛋白的表达水平增高。Oncomine数据库结果显示,多个肺癌基因芯片中RAD21的m RNA水平明显增高(P<0.01)。Kaplan-Meier生存曲线结果显示,RAD21的表达水平与肺癌患者的生存时间存在相关性(P<0.05);基因集富集分析显示,RAD21主要富集于细胞凋亡与细胞周期调控相关的信号通路。结论肺癌中RAD21的表达水平可作为判断肺癌患者预后的生物标志物。RAD21可能通过调控细胞周期和凋亡参与肺癌的发生和发展。Objective To detect the expression levels of RAD21 （S.Pombe RAD21 homolog） in lung cancer, and assess its clinical significance and potential functions. Methods The expression level of RAD21 in lung cancer was determined by Western blot and immunohistochemistry meth- ods ; the RAD21 mRNA expression in lung cancer was analyzed through Oncomine database ; the relationship between the RAD21 expression levels and prognosis in patients with lung cancer was studied by Kaplan Meier curve ; the RAD21 functions in lung cancer were predicted by using gene set enrichment analysis. Results Compared with adjacent normal tissue, the protein level of RAD21 in lung tissues were upregulated. The data from Oncomine database showed that RAD21 mRNA levels were significantly higher than the normal control groups in multiple lung cancer datasets （P 〈 0.01 ） ; in addition, Kaplan-Meier survival curves showed that the RAD21 expression levels was correlated with the survival of patients with lung can- cer （P 〈 0.05） ; gene set enrichment analysis indicated that RAD21 was mainly enriched in apoptotic signaling pathways and cell cycle regulation gene sets. Conclusion The downregulated RAD21 could be used as a biomarker to assess the prognosis of lung cancer patients. In addition, RAD21 may be involved in the regulation of cell cycle and apoptosis, which affects the development and progression of lung cancer.

关 键 词：肺癌 RAD21 预后 免疫组织化学 

分 类 号：R34[医药卫生—基础医学]