不同葡萄糖浓度对体外培养人牙龈成纤维细胞增殖的影响  

Effects of glucose at different concentrations on proliferation of in-vitro cultured human gingival fibroblasts

作　　者：常文晓[1] 安阳[1] 张含中[2] 李振强[1] 高林琳[3] 张楠[1] 

机构地区：[1]山西医学科学院山西大医院口腔科,太原030032 [2]武汉市中心医院口腔科 [3]山西医学科学院山西大医院内分泌科,太原030032

出　　处：《中国药物与临床》2016年第2期164-165,共2页Chinese Remedies & Clinics

基　　金：山西省卫生计生委科研项目(2014006)

摘　　要：目的探讨不同葡萄糖浓度下人牙龈成纤维细胞的生长情况。方法采用体外模型,观察在不同葡萄糖浓度(5.5,7,8.8,10,15及20 mmol/L)细胞培养液中人牙龈成纤维细胞在24 h、3 d、7 d时细胞形态及生长增殖情况。结果当葡萄糖浓度低于8.8 mmol/L时,细胞形态规则,对照组、A组、B组及C组间细胞增殖无明显差异;当浓度高于10 mmol/L时,D组、E组及F组细胞形态呈现不规则,各组增殖能力明显受到抑制。结论高浓度的葡萄糖能够影响人牙龈成纤维细胞的形态,并可抑制其增殖。Objective To investigate the growth of human gingival fibroblasts under different concentrations of glucose. Methods The in vitro models were established. The morphology and proliferation of the human gingival fibroblasts in different concentrations of glucose solution（5.5, 7, 8.8, 10, 15 and 20 mmol/L） at 24 h, 3 d and 7 d were determined. Results When the glucose concentration was lower than 8.8 mmol/L, the cell morphology was regular,and there was no significant difference in the proliferation among the control group, group A, group B, and group C.When the glucose concentration was higher than 10 mmol/L, the cell morphology in group D, group E and group F was irregular, and cell proliferation in each group was significantly inhibited. Conclusion High concentration of glucose may affect the morphology of human gingival fibroblasts and inhibit their proliferation.

关 键 词：葡萄糖 成纤维细胞 细胞增殖 

分 类 号：R587.1[医药卫生—内分泌] R783.6[医药卫生—内科学]