出入境人员丙型肝炎病毒感染者miRNA-122表达变化研究  

Study on change of miRNA-122 expression in exit-entry people with hepatitis C virus infection

作　　者：史咏梅[1] 冯子力[1] 伍碧梅[1] 唐明慧[1] 王琪[1] 涂承宁[1] 谭华[1] 叶立青[1] 

机构地区：[1]珠海出入境检验检疫局国际旅行卫生保健中心,广东珠海519020

出　　处：《检验医学与临床》2016年第6期738-740,共3页Laboratory Medicine and Clinic

基　　金：珠海出入境检验检疫局科技计划项目(ZH2012-2)

摘　　要：目的探讨珠海口岸出入境人员丙型肝炎病毒(HCV RNA)感染者外周血miRNA-122表达的变化及其对丙型肝炎患者的临床意义。方法收集96例丙型肝炎IgG抗体初筛阳性患者外周血,定量检测HCV RNA水平。对HCV RNA阳性患者检测miRNA-122、丙氨酸氨基转移酶(ALT)及检查肝脏超声影像学改变,并进行相关性分析。结果 96例HCV IgG阳性患者检测出49例HCV RNA阳性,占60.9%;两组患者miRNA-122表达水平差异有统计学意义(P<0.01);与ALT呈正相关,与HCV RNA无相关性。B超检查发现11例合并轻度脂肪肝,合并脂肪肝患者miRNA-122表达水平高于无脂肪肝患者。结论 HCV阳性患者循环miRNA-122表达可能与病毒复制无关,而是反映肝脏组织活动性损害和病变严重性的指标。Objective To investigate the change of peripheral blood miRNA-122 expression in exit-entry people with hepatitis C virus（HCV）infection at Zhuhai port and its significance to the patients with hepatitis C.Methods The peripheral blood was collected from 96 cases of HCV IgG antibody primary screening positive for quantitatively detecting the HCV RNA level.Then the individuals of HCV RNA positive were detected miRNA-122,ALT and hepatic B-ultrasonic examination was performed.Furthermore the correlation analysis was conducted.ResultsAmong 96 cases of HCV IgG positive,49cases（60.9%）were HCV RNA positive;the expression level of miRNA-122 had statistical difference between the experimental group wand the control group（P〈0.01）;the correlative analysis showed that there was a positive correlation between the expression of MiRNA-122 and ALT and no significant correlation between the expression of miRNA-122 and HCV RNA was found.Eleven cases of complicating mild fat liver were found by B-ultrasound and the expression level of miRNA-122 in the patients with complicating fat liver was higher than that in the patients with non-fat liver.Conclusion The circulating miRNA-122 expression may be irrelevant with HCV virus replication and is an indicator reflecting the active liver damage and lesion severity.

关 键 词：miRNA-122 丙型肝炎 脂肪肝 

分 类 号：R512.63[医药卫生—内科学]