针刺对DMBA诱导的乳腺癌大鼠ER、PR表达的影响  

Effect of Acupuncture on ER and PR Expressions in Rats with DMBA-induced Mammary Cancer

作　　者：曹加伟 黄梅[1] 严晶[1] 徐金龙[1] 杨增荣[1] 黄昆[1] 朱珠[1] 赵荣[1] 

机构地区：[1]云南中医学院,昆明650000

出　　处：《上海针灸杂志》2016年第3期344-348,共5页Shanghai Journal of Acupuncture and Moxibustion

基　　金：云南省中青年学术技术带头人后备人才项目(2012HB034);云南省教育厅科学研究基金项目(2015J103)

摘　　要：目的观察针刺足三里、三阴交、膻中对二甲基苯蒽（DMBA）诱导的乳腺癌大鼠乳腺组织中雌激素受体（ER）、孕激素受体（PR）表达的影响。方法将120只6-8周龄的SD雌性大鼠随机分为模型组90只和空白组30只。模型组采用DMBA进行灌胃造模,空白组给予等剂量的芝麻油灌胃。造模15星期后,将模型组大鼠随机分为治疗组和对照组。治疗组采用针刺足三里、三阴交、膻中治疗,对照组和空白组不采用任何治疗,仅给予相同的抓放。针刺治疗结束后（第27星期）,采用电化学发光免疫方法,取腹腔静脉血做血清肿瘤标志物检测;计数肿瘤包块数量并记录形态,取包块组织,用精度为1 mm的游标卡尺测量包块的高度、最大直径和相垂直的直径;采用Olympus光学显微镜下观察肿瘤组织病理学变化及ER、PR的阳性面积及平均光密度。结果治疗组治疗后乳腺肿瘤平均数目和平均体积与对照组和空白组比较,差异均具有统计学意义（P〈0.01,P〈0.05）。对照组治疗后乳腺肿瘤平均数目和平均体积与空白组比较,差异均具有统计学意义（P〈0.01）。治疗组和对照组治疗后各项肿瘤标志物（CA724、CA125、CA199、AEP、CA15-3、CEA及CA50）浓度与空白组比较,差异均具有统计学意义（P〈0.01,P〈0.05）。治疗组治疗后CA15-3浓度与对照组比较,差异具有统计学意义（P〈0.01）。治疗组治疗后ER、PR阳性面积和平均光密度与对照组和空白组比较,差异均具有统计学意义（P〈0.01）。对照组治疗后ER、PR阳性面积和平均光密度与空白组比较,差异均具有统计学意义（P〈0.01）。结论针刺能够减少DMBA诱导的大鼠乳腺肿瘤的发生（包括肿瘤数目及肿瘤体积的大小）,其作用机制可能与降低CA724、CA125、CA199、CA15-3、AEP、CEA、CA50等肿瘤标志物的浓度,尤其是降低CA15-3的浓度,减少ER、PR的阳性面积和平均光密度相关。Objective To investigate the effect of acupuncture at Zusanli, Sanyinjiao and Danzhong on mammary estrogen receptor（ER） and progesterone receptor（PR） expressions in rats with dimethylbenzanthracine（DMBA）-induced mammary cancer. Methods One hundred and twenty female SD rats aged 6-8 weeks were randomized into a model group of 60 rats and a blank group of 30 rats. The model group received an oral gavage of DMBA for model making. The blank group received an oral gavage of equal volume of sesame oil. At 15 weeks after model making, the model group of rats was randomized into treatment and control groups. The treatment group received acupuncture at Zusanli, Sanyinjiao and Danzhong, and the control and blank groups, only the same grasp and release. After the completion of acupuncture treatment（twenty-seventh week）, abdominal venous blood was taken and serum tumor markers were determined by electrochemiluminescence immunoassay. Tumor masses were counted and their shapes were recorded. The mass was taken and its height, maximum diameter and vertical diameter were measured using a 1 mm precision vernier caliper. Pathological changes in tumor tissues, and ER and PR positive areas and mean optical densities were observed under an Olympus optical microscope. Results There were statistically significant post-treatment differences in the average number and volume of mammary tumors between the treatment group and the control or blank group（p〈0.01, p〈0.05） and between the control and blank groups（p〈0.01）. There were statistically significant post-treatment differences in the concentrations of various tumor markers（CA724, CA125, CA199, AEP, CA15-3, CEA and CA50） between the treatment or control group and the blank group（p〈0.01, p〈0.05） and between the control and blank groups（p〈0.01）. There was a statistically significant post-treatment difference in CA15-3 concentration between the treatment and control groups（p〈0.01）. There were statistically significant post-treatment diffe

关 键 词：针刺疗法 乳腺肿瘤 穴 足三里 穴 三阴交 穴 膻中 雌激素受体 孕激素受体 二甲基苯蒽 CA15-3 大鼠 

分 类 号：R2-03[医药卫生—中医学]