RT-PCR检测SALL4基因骨髓增生异常综合征的表达  

Detection of SALL4 mRNA expression in myelodysplastic syndrome by RT-PCR

作　　者：武广海 田黎明[1] 于广晴[1] 张纯[1] 袁嘉怡[1] 申福国 

机构地区：[1]佳木斯大学附属第一医院,黑龙江佳木斯154003 [2]鹤岗市人民医院急救中心,黑龙江鹤岗154100

出　　处：《黑龙江医药科学》2016年第2期96-98,共3页Heilongjiang Medicine and Pharmacy

摘　　要：目的:探讨骨髓增生异常综合征(MDS)患者骨髓中SALL4mRNA表达及其临床意义。方法:通过RT-PCR方法分别检测13例难治性贫血患者(MDS-RA)、27例难治性贫血伴原始细胞增多患者(MDS-RAEB)和11例正常对照组骨髓单个核细胞(BMMNC)中SALL4mRNA表达情况,并比较MDS-RA和MDS-RAEB之间的表达差异。结果:MDS患者骨髓中SALL4 mRNA表达均明显高于正常对照,且MDS-RAEB表达量高于MDS-RA,差异有统计学意义(P<0.01)。结论:SALL4mRNA基因可能参与了MDS的发病,MDS-RA和MDS-RAEB之间的SALL4基因表达差异可能提示2者的发病机制的不同。Objective： To investigate the expression of SALL4 mRNA and its clinical significance on myelodysplastic syndrome（ MDS） patients with bone marrow SALL4 mRNA Expression and clinical significance. Methods：RT- PCR,13 cases were detected in patients with refractory anemia（ MDS- RA）.,27 patients with refractory anemia with excess blasts in patients（ MDS- RAEB） and 11 cases of normal bone marrow mononuclear cells（ BMMNC） in SALL4 mRNA expression and compared expression differences between MDS- RA and MDS-RAEB betwee. Results： SALL4 mRNA expression in MDS patients were significantly higher than that in the control group.,a And MDS- RAEB higher expression of MDS- RA, the difference was statistically significant（ P〈0. 01）. Conclusion： SALL4 mRNA gene may be involved in the pathogenesis of MDS,SALL4 gene expression between differences MDS- RA and MDS- RAEB may suggest different pathogenesis. between two persons may suggest different pathogenesis.

关 键 词：SALL4mRNA 骨髓增生异常综合征 RT-PCR 

分 类 号：R551.3[医药卫生—血液循环系统疾病]