不同产地山药中总多糖及尿囊素的含量分析  

Content analysis of polysaccharide and allantoin of Dioscorea opposite Thunb. from different habitats

作　　者：闫沛沛 杨文华[1] 曹俊岭[2,3] 李朝峰[1] 戚姝娅 赵华叶 樊冬鹤 张玉君[1] 张梦捷[1] 

机构地区：[1]北京中医药大学中药学院,硕士研究生100029 [2]北京中医药大学东直门医院药学部 [3]中国中医科学院中药资源中心

出　　处：《环球中医药》2016年第3期295-298,共4页Global Traditional Chinese Medicine

基　　金：中央本级重大增减支项目(2060302);国家自然科学基金(81130070);江苏高校协同创新项目

摘　　要：目的对比河南产地和河北产地山药的品质,寻找优良品种。方法总多糖含量采用苯酚硫酸法测定;尿囊素含量采用高效液相色谱法测定,色谱条件:色谱柱:资生堂SPOLAR C_(18)(4.6 mm×250 mm,5μm),流动相:乙腈-水(2:98),流速:0.5 m L/min,柱温25℃,检测波长:224 nm。结果总多糖含量(%)河南产地山药中为(14.65±1.43)%,河北产地山药中为(7.78±2.83)%,二者有显著性差异(P<0.01);在尿囊素含量上二者没有显著性差异(P>0.05)。结论河南产地的山药中含有较高的总多糖,可能药用价值更高,品质更优,是值得关注的优良品种。Objective To compare the quality of the Dioscorea opposite Thunb. from Henan and Hebei by determining the content of polysaccharide and allantoin. Methods Phenol-sulfuric acid method was used to determine the content of polysaccharide. Allantoin content was determined by high performance liquid chromatography. SPOLAR C18（ 250 mm × 4. 6mm,5μm） was used. Mobile phase was acetonitrilewater（ 2： 98）. The flow rate was 0. 5 m L / min. The column temperature was set at 25℃. The detection wavelength was set at 224 nm. Results The polysaccharide content（ %） in the Dioscorea opposite Thunb.from Henan was（ 14. 65 ± 1. 43）,while the Hebei was（ 7. 78 ± 2. 83）. There was significant statistical difference between the two varieties（ P〈0. 01）. There was no significant difference of the allantoin content between the varieties（ P〉0. 05）. Conclusion The Dioscorea opposite Thunb. from Henan contains higher polysaccharide,which might have higher medicinal value and better quality,deserves paying close attention as a good variety.

关 键 词：总多糖 尿囊素 河南产地山药 河北产地山药 

分 类 号：R284[医药卫生—中药学]