补肾壮阳胶囊对精神分裂症模型大鼠海马胶质细胞源性神经营养因子表达的影响  

Effects of Warm--Supplementing Kidney Yang Capsules on the express of gliai cell--line derived neurotro- phic factor in the hippocampus of schizophrenia model rats

作　　者：韩娇[1] 陈振华[1] 王高华[1] 秦丽[1] 

机构地区：[1]武汉大学人民医院精神卫生中心,430000

出　　处：《神经疾病与精神卫生》2016年第1期77-80,共4页Journal of Neuroscience and Mental Health

摘　　要：目的探讨补肾壮阳胶囊（WSKY）对地卓西平马来酸盐（MK801）建立的精神分裂症模型大鼠海马胶质细胞源性神经营养因子（GDNF）表达的影响。方法将40只6周龄SD雄性大鼠随机分为3组：对照组（生理盐水腹腔注射＋生理盐水灌胃）、模型组（MK801腹腔注射＋生理盐水灌胃）及WSKY＋MK801组（MK801腹腔注射＋WSKY灌胃，而根据WSKY剂量的不同又分为3个亚组）；各组相应处理两周后运用WesternBlot和RT-PCR技术分别检测各组大鼠海马区GDNF蛋白及mRNA的表达。结果与对照组相比较，模型组的GDNF蛋白及mRNA的表达下降，差异有统计学意义（P〈0．05）；而与模型组相比，wSKY＋MK801组中较高剂量WSKY可致GDNF蛋白及其mRNA的表达增加，差异有统计学意义（P〈0．05）。结论MK801可致大鼠海马GDNF表达减少，而补肾壮阳胶囊可上调大鼠海马GDNF的表达。Objective To investigate the influence of Warm--Supplementing Kidney Yang cap- sules（WSKY） on the express of glial cell--line derived neurotrophic factor（GDNF） in the hippocampus of schizophrenia model rats. Methods Fourty male Sprague--Dawley（SD） rats were randomly divided into three groups： control group（saline i. p. ＋ saline）, model group（MK801 i. p. ＋ saline）,WSKY＋MK801 group（MK801 i. p. ＋ WSKY,separated into three subgroups according to the different doses of WSKY） ; After two weeks when every group got the corresponding treatment, using Western blot and RT- PCR to detect the express of GDNF and it＇s mRNA in hippocampus. Results Compared with control group,the express of GDNF and it＇s mRNA in model group decreased, there was significant difference （P 0.05）; While compared with model group, the high dose WSKY in WSKY＋MK801 group can increase the express of GDNF and it＇s mRNA, there was significant difference （P 〈 0.05）. Conclusions MK801 can decrease the express of GDNF in hippocampus,but WSKY raise it.

关 键 词：精神分裂症 胶质细胞源性神经营养因子 补肾壮阳胶囊 

分 类 号：R651.2[医药卫生—外科学]