喉癌干细胞的新型培养纯化方法及临床应用前景  

作　　者：段广亮[1] 王凯峰[1] 戴辉萍 唐婷婷[1] 丁飞[1] 

机构地区：[1]杭州师范大学附属医院,310015

出　　处：《浙江临床医学》2016年第4期602-604,共3页Zhejiang Clinical Medical Journal

摘　　要：目的通过舍生长因子的无血清培养基（SFM）培养Hep-2喉癌细胞，分离并扩增肿瘤干细胞，为进一步制备放疗增敏剂做基础研究。方法含生长因子的无血清培养基（SFM）培养Hep-2喉癌细胞，流式细胞仪检测细胞中侧群细胞的比例。当侧群细胞比例达25％时，收集全部细胞球，FAcs筛选出CD133^＋、CD44^＋细胞作为喉癌干细胞。结果成功培养出侧群细胞，流式细胞仪检测其比例达26．2％，FACS筛选出CD133^＋CD44^＋细胞的比例均〉91％。结论成功培养出高浓度喉癌干细胞。Objective To explore a new culturing method for Hep-2 Laryngeal Cancer Cell by menas of the SFM of containing growth factor separate and to amplify cancer stem cell. Methods Culturing Hep-2 Laryngeal Cancer Cell by menas of the SFM of containing growth factor, FACS was used to detect the ratio of side population. When the ratio of side population expressed reached 25%, the whole cell balls were collected. FACS was used to screen out CD133^＋ CD44^＋cells, which were laryngeal cancer stem cell. Results The side population were successful cultured and reached the ratio of 26.2% by detection of FACS; Overtop 91% of CD133^＋ CD44^＋ cells were screened out by FACS. Conclusion High ration of laryngeal cancer stem cell can be successfully cultured.

关 键 词：无血清培养基 重组表皮生长因子 成纤维生长因子 侧群细胞 

分 类 号：R392-33[医药卫生—免疫学]