硝酸铵对红掌“粉冠军”愈伤组织诱导和芽分化的影响  

Effects of NH_4NO_3 on Callus Induction and Bud Differentiation of Anthurium Andraeanum Pink Champion

作　　者：侯艳霞[1] 杨婧[1] 郝瑞芳 杨玉芳[1] 张文鑫[1] 

机构地区：[1]山西林业职业技术学院园艺系,山西太原030009

出　　处：《农业技术与装备》2016年第1期12-13,共2页Agricultural Technology & Equipment

基　　金：山西林业职业技术学院院级科研教改项目(201314)

摘　　要：以红掌"粉冠军"的叶片为外植体,研究不同含量硝酸铵对"粉冠军"愈伤组织诱导和芽分化的影响。结果表明,当硝酸铵含量为200 mg/L时,最适合"粉冠军"叶片愈伤组织的诱导和分化。叶片愈伤组织诱导的最佳培养基为1/2MS+6-BA 1 mg/L+2,4-D0.2 mg/L+蔗糖20 g+琼脂7 g+N H4N O3(200 mg/L);愈伤组织分化的最佳培养基为1/2MS+6-BA1 mg/L+2,4-D0.2 mg/L+蔗糖20 g+琼脂7 g+土豆15 g/L+N H4N O3(200 mg/L)。With the leaves as explants, the article studied the effects of NH4NO3 on callus induction and bud differentiation of anthurium andraeanum ＇pink champion＇. The results showed that when the content of NH4NO3 was 200 mg/L, the induction and differentiation of the leaves callus were the best. The optimal culture medium for callus induction was 1/2MS ＋6-BA 1.0 mg/L ＋ 2, 4-D 0.2 mg/L ＋ sucrose 20 g ＋ agar 7g ＋NH4NO3（200 mg/L）. The best culture medium for bud differentiation was 1/2MS ＋ 6-BA 1.0 mg/L ＋ 2,4-D 0.2 mg/L ＋ sucrose 20 g ＋ agar 7g ＋ potatoes 15 g / L ＋ NH4NO3（200mg/L）.

关 键 词：粉冠军 愈伤组织诱导 芽分化 硝酸铵 

分 类 号：S682.14[农业科学—观赏园艺]