再生障碍性贫血患者血清Th1/Th2细胞及相关细胞因子和T细胞亚群变化及意义  

Changes and significances of Th1/Th2related cytokines and T cell subgroup in aplastic anemia patients

作　　者：张红霞[1] 吴广胜[1] 郭蔚玲[1] 

机构地区：[1]石河子大学医学院第一附属医院血液风湿科,新疆石河子832000

出　　处：《海南医学院学报》2016年第10期1024-1026,1030,共4页Journal of Hainan Medical University

基　　金：新疆维吾尔自治区省级攻关项目(2015272B171)~~

摘　　要：目的:探讨再生障碍性贫血(AA)患者血清Th1/Th2细胞及相关细胞因子和T细胞亚群变化及意义。方法:选择87例AA患者作为研究对象,设为观察组,并根据病情分为急性组(n=21)和慢性组(n=66),另选择30例健康志愿者作为对照组,酶联免疫吸附法检测各组Th1细胞因子干扰素-γ(IFN-γ)和Th2细胞因子白细胞介素-4(IL-4),流式细胞仪检测各组Th1/Th2细胞和T细胞亚群。结果:观察组外周血Th1细胞、Th1细胞/Th2细胞水平均显著高于对照组,差异有统计学意义(P<0.05),其中急性组外周血Th1细胞、Th1细胞/Th2细胞水平显著高于慢性组,差异有统计学意义(P<0.05);观察组外周血IFN-γ、IL-4以及IFN-γ/IL-4均显著高于对照组,差异有统计学意义(P<0.05),其中急性组外周血IFN-γ、IL-4以及IFN-γ/IL-4均显著高于慢性组,差异有统计学意义(P<0.05);观察组外周血CD8^+水平显著高于对照组,CD4^+、CD4^+/CD8^+水平显著低于对照组,差异均有统计学意义(P<0.05)。急性组外周血CD8^+水平显著高于慢性组,CD4^+及CD4^+/CD8^+水平显著低于慢性组,差异均有统计学意义(P<0.05)。结论:T细胞亚群失衡以及Th1偏移在AA的发病中扮演重要角色,且能一定程度反映疾病的状态。Objective：To investigate the changes and significances of Th1/Th2,related cytokines and T cell subgroup in aplastic anemia（AA）patients.Methods：A total of 87 cases AA patients was chosen and set as observation group,and they were divided into acute group（n=21）and chronic group（n=66）according to the condition.Another 30 cases of healthy volunteers was chosen as control group,detected the Th1 cytokine interferon-γ（IFN-γ）and Th2 cytokines interleukin-4（IL-4）with nzyme-linked immunosorbent method,detected the Th1/Th2 cells and T cells subgroup with flow cytometry instrument between groups.Results：The Th1 cells and Th1/Th2 cells in observation group were significantly higher than control group.and the Th1 cells and Th1/Th2 cells in acute group were significantly higher than chronic group（P〈0.05）;The IFN-γ,IL-4and IFN-γ/IL-4in observation group were significantly higher than that of control group,and the IFN-γ,IL-4and IFN-γ/IL-4in acute group were significantly higher than chronic group（P〈0.05）;The blood CD4＋,CD4＋/CD8＋in observation group were significantly lower than the control group,CD8＋was significantly higher than the control group,and the blood CD4＋,CD4＋/CD8＋in acute group were significantly lower than the chronic group,CD8＋was significantly higher than the chronic group,all the difference was statistically significant（P〈0.05）.Conclusion：T cell subgroup imbalances and Th1 migration plays the important role in the onset of AA,and may relate to a certain degree of disease state.

关 键 词：再生障碍性贫血 TH1/TH2 细胞因子 T细胞亚群 

分 类 号：R556.5[医药卫生—血液循环系统疾病]