清热涤痰解痉方对冠状动脉痉挛小鼠血清ox-LDL、hs-CRP及ET-1的影响  

Effects of Qingre Ditan Jiejing Fang( 清热涤痰解痉方) on Serum ox-LDL,hs-CRP and ET-1 of Coronary Artery Spasm Mice

作　　者：王微微[1] 郭双庚[2] 杜峰[3] 齐建兴[2] 

机构地区：[1]河北医科大学 [2]河北省中医药研究院 [3]石家庄市第一医院

出　　处：《中医杂志》2016年第6期520-523,共4页Journal of Traditional Chinese Medicine

摘　　要：目的观察清热涤痰方对垂体后叶素诱发高脂血症小鼠冠状动脉痉挛的影响,并探讨其可能的作用机制。方法将140只C57BL/6J小鼠随机分为正常组、模型组、合心爽组、阿托伐他汀组和中药高、中、低剂量组,每组20只。除正常组外,其余各组均给予高脂饲料饲养4周。中药高、中、低剂量组分别给予清热涤痰解痉方颗粒剂7.2、3.6、1.8 g/(kg·d),合心爽组给予合心爽0.04 g/(kg·d),阿托伐汀组给予阿托伐他汀钙片0.013 g/(kg·d),正常组和模型组予同体积0.5%羧甲基纤维素钠溶液,每日1次,连续4周。末次给药后1 h,腹腔注射垂体后叶素注射液60 U/kg建立冠状动脉痉挛模型。记录注射前10 min和注射后5、10、15 min时点Ⅱ导联心电图,计算T波位移值,试验结束后检测血清氧化型低密度脂蛋白(ox-LDL)、高敏C反应蛋白(hs-CRP)、内皮素1(ET-1)水平。结果模型组腹腔注射垂体后叶素后5、10、15 min时,小鼠心电图T波位移值较正常组有明显升高(P<0.05);与模型组比较,合心爽组、阿托伐他汀组和中药高、中、低剂量组在5、10、15 min时均能降低T波位移值(P<0.05);与阿托伐他汀组比较,中药高、中剂量组在5、10 min时T波位移值显著降低,中药高剂量组在15 min时T波位移值亦明显降低(P<0.05)。与正常组比较,其余各组血清ox-LDL、hs-CRP、ET-1含量均明显升高(P<0.05或P<0.01)。与模型组比较,中药高剂量和阿托伐他汀组ox-LDL含量明显降低,各给药组hs-CRP含量均明显降低,合心爽组和中药高剂量组ET-1含量明显降低(P<0.05)。结论清热涤痰解痉方能有效缓解垂体后叶素诱发高脂血症小鼠冠状动脉痉挛,其作用机制可能与降低血清ox-LDL、hs-CRP、ET-1水平有关。Objective To observe effects of Qingre Ditan Jiejing Fang（ 清热涤痰解痉方） on mice with hyperlipemia and coronary artery spasm induced by pituitrin and study the possible mechanism. Methods Totally 140C57 BL /6J mice were randomly divided into normal group,model group,diltiazem group,atorvastatin group and Chinese medicine high,medium and low dose group,with 20 mice in each group. Except for the normal group,the rest groups were given high-fat fodder to feed for 4 weeks. The Chinese medicine high,medium and low dose group were respectively given granules of Qingre Ditan Jiejing Fang,7. 2,3. 6,1. 8 g /（ kg·d）. The diltiazem group was given diltiazem,0. 04 g /（ kg·d）. The atorvastatin group was given atorvastatin calcium tablet,0. 013 g /（ kg · d）. The normal group and the model group were given 0. 5% sodium carboxymethyl cellulose solution with same volume,once a day for four weeks. One hour after the last given treatment,the mice were given intraperitoneal injection with pituitrin 60 U / kg to establish model of coronary artery spasm. The researchers recorded Ⅱ-lead electrocardiogram at the time that 10 min before and 5,10,15 min after injection and counted displacement of T wave. The researchers detected level of serum oxidized low density lipoprotein（ ox-LDL）,high-sensitivity C-reactive protein（ hs-CRP） and endothelin-1（ ET-1） after test. Results At the time that 5,10,15 min after intraperitoneal injection with pituitrin,in the model group,displacement of T wave in electrocardiogram significantly increased compared with the normal group（ P〈0. 05）; Compared with the model group,the diltiazem group,the atorvastatin group and the Chinese medicine high,medium and low dose group at above three time all can lower displacement of T wave（ P〈0. 05）; Compared with the atorvastatin group,displacement of T wave significantly decreased in the Chinese medicine high and medium dose group at the 5,10 min and the Chinese medicine high dose group at the 15 min（ P〈0. 05）.

关 键 词：清热涤痰解痉方 冠状动脉痉挛 高脂血症 氧化型低密度脂蛋白 高敏C反应蛋白 内皮素1 

分 类 号：R285.5[医药卫生—中药学]