菠萝蛋白酶对家兔肾上腺素型肺水肿的治疗效果  

Effects of bromelain on rabbits with pulmonary edema induced by adrenaline

作　　者：乔红[2] 陈谱[2] 孙学娇[2] 朱冰清[1] 李晓芳[2] 段静雨[1] 张春平[1] 

机构地区：[1]徐州医学院药学院,江苏省新药研究与临床药学重点实验室,江苏徐州221004 [2]徐州医学院临床医学系,江苏徐州221004

出　　处：《徐州医学院学报》2016年第1期34-36,共3页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金（31300222）;江苏省自然科学基金（BK20130214）;江苏省高校自然科学研究项目（13KJB180025）;2014年高等学校大学生实践创新训练计划项目（201410313009,201410313009Z）;徐州医学院优秀人才科研启动基金

摘　　要：目的：研究菠萝蛋白酶对家兔肾上腺素型肺水肿的治疗效果。方法将家兔随机分为4组，空白对照组（A）、肾上腺素致病组（B）、阳性对照呋塞米治疗组（C）和菠萝蛋白酶治疗组（D）。4组均静脉滴注生理盐水， B、C和D组再给予0．75 ml／kg肾上腺素建立肺水肿模型，然后C组和D组分别给予呋塞米和菠萝蛋白酶进行治疗。记录各小组家兔体征、心率和呼吸频率的变化，并比较肺系数和肺组织石蜡切片苏木精－伊红染色结果。结果 B组成功建立肺水肿模型。与B组相比，D组口唇未见紫绀，呼吸频率稍有加快，心率先加快后缓慢，肺系数显著降低（P＜0．05）。取肺后见肺组织水肿症状已明显减轻，病理见肺间隔轻微破坏，淤血现象已基本消失。 C组与D组无显著性差异。结论菠萝蛋白酶对家兔肾上腺素型肺水肿有治疗效果。Objective To investigate the therapeutic effects of bromelain on rabbits with pulmonary edema induced by adrenaline .Methods The rabbits are randomly divided into four groups：a control group （ A） , an epinephrine group （B）, a furosemide treatment group （C）, and a bromelain treatment group （D）.All the groups were intravenously in-fused with normal saline.Furthermore, Groups B, C, and D were administrated with epinephrine （0.75 ml/kg） to es-tablish a rabbit model of pulmonary edema .Then, Groups C and D were treated with furosemide and bomelain , respec-tively.The changes in physical signs , heart rate and respiratory frequency were recorded , while pulmonary coefficient and the results of H-E staining were compared .Results A rabbit model of pulmonary edema was successfully established in Group B.Compared with Group B , Group D did not show cyanosis in the lip but produced slightly faster respiratory frequency.An increased heart rate was also found in Group D , which was then decreased .The pulmonary coefficient of Group D was remarkably reduced （P〈0.05）.The lung tissues were taken from Group D, which indicated markedly re-lieved edema .According to pathological observation , Groups D showed the slightly broken lung septum , without any con-gestion.There is no significant difference between Group C and Group D .Conclusion Bromelain produces therapeutic effects on rabbits with pulmonary edema induced by adrenaline .

关 键 词：肺水肿 肾上腺素 呋塞米 菠萝蛋白酶 

分 类 号：R587.1[医药卫生—内分泌]