鹿特异性复合麻醉剂对大鼠大脑皮层Na^+-K^+-ATP酶活性的影响  

Effects of Specificity Anesthetic for Deer on Na^+-K^+-ATPase Activity in Cerebral Cortex of Rats

作　　者：冯雪[1] 付连军[1] 林博[1] 常乃元 李京乘 李闻轩[1] 尹柏双[1] 

机构地区：[1]吉林农业科技学院动物科技学院,吉林吉林132101

出　　处：《湖北农业科学》2016年第4期964-965,970,共3页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31302150);吉林省科技厅发展计划项目(20140204062NY);吉林省教育厅"十二五"科学技术项目(吉教科合字2014379);长白山动植物资源保护与利用省级重点实验室基金项目(吉农院合字2012701;2013S029);吉林农业科技学院博士启动基金项目(吉农院合字2012307);吉林农业科技学院种子基金项目(吉农院合字2013905);吉林农业科技学院大学生科技创新项目(吉农院合字2014094)

摘　　要：通过测定鹿特异性复合麻醉剂麻醉下大鼠大脑皮层Na^+-K^+-ATP酶活性的变化,探讨了其麻醉与大鼠大脑皮层Na^+-K^+-ATP酶的关系。将32只纯种SD大鼠随机分成对照组、诱导组、麻醉组和催醒组,对照组大鼠按30 m L/kg体重腹腔注射生理盐水,其余各组按30 mg/kg体重腹腔注射鹿特异性复合麻醉剂,于各时间点冰上采集各组大鼠大脑皮层,采用比色法测定其Na^+-K^+-ATP酶活性。结果显示,药物作用后大鼠大脑皮层Na^+-K^+-ATP酶活性显著低于对照组(P<0.05或P<0.01)。该结果提示大鼠大脑皮层Na^+-K^+-ATP酶活性变化与鹿特异性复合麻醉剂的麻醉作用具有相关性。This experiment was aimed to determine the activity of Na^＋-K^＋-ATPase in rats＇ cerebral cortex region and investigate the relationship between specificity anesthetic for deer and Na^＋-K^＋-ATPase in cerebral cortex. 32 SD rats were randomly divided into control group, induction group, anesthesia group and the wake group. Rats of the control were injected with saline（30 m L / kg）,rats of the other group were injected with drug（30 mg / kg）. The samples of cerebral cortex in different period were separated and the activities of Na^＋-K^＋-ATPase were determined by using colorimetry methods. The results showed that the activities of Na^＋-K^＋-ATPase in cerebral cortex after administration were decreased obviously compared with that in the control group（P〈0.05 or P〈0.01）. It indicated that the changes of the activity of Na^＋-K^＋-ATPase in the cerebral cortex were correlated with the specificity anesthetic for deer.

关 键 词：鹿特异性复合麻醉剂 大鼠 大脑皮层 NA+-K+-ATP酶 

分 类 号：S859.791[农业科学—临床兽医学]