一株碱性果胶酶产生菌的筛选、鉴定及发酵条件优化  

Screening,Identification of Alkaline Pectinase Producing Strain and Optimization of Its Fermentation Conditions

作　　者：周熠[1] 黄河[1] 冯波[1] 林元山[1] 

机构地区：[1]湖南农业大学生物科学技术学院,长沙410128

出　　处：《湖北农业科学》2016年第4期992-996,共5页Hubei Agricultural Sciences

基　　金：湖南省大学生创新项目(DFCXS201203)

摘　　要：采用平板分离结合刚果红染色法从野生苎麻土壤中分离得到1株果胶酶产生菌Lys-5304。经形态观察和16S r DNA测序鉴定,该菌株鉴定为欧文氏菌(Erwinia amylovora)。通过单因素试验和响应面分析,该菌产果胶酶的最优条件为:葡萄糖3.0 g/100 m L,菜子粕0.2 g/100 m L,接种量1.5 m L,培养基起始p H 8.0,摇床转速180 r/min,发酵周期为72 h,培养温度为37℃。在此条件下,该菌的果胶酶活力达到1 021.33 U/m L,具有一定应用潜力。A pectinase producing strain LYS-5304 was screened from wild ramie soil by methods of plate isolation with Congo red staining.Strain LYS-5304 was identified as Erwinia carotovora according to morphological observation and 16 S r DNA sequencing.Furthering studying,by means of single factor experiment and response surface analysis, showed that optimum conditions for pectinase production were as follows： Culture media containing glucose 3.0%,rapeseed meal 0.2%,inoculation volume 1.5%,initial p H of 8.0,at 37 ℃ shaking at 180 r / min for 72 h. The activity pectinase of LYS-5304 reached 1 021.33 U /m L under these conditions,and its indicated some potential applications.

关 键 词：果胶酶 欧文氏菌(Erwinia amylovora) 发酵 响应面分析 

分 类 号：Q93-33[生物学—微生物学]