微囊化胰岛移植联合肾动脉重建缓解糖尿病肾病大鼠早期肾脏病变  

Transplantation of microencapsulated islets combined with renal arterial reconstruction to alleviate the early stage renal pathology in diabetic nephropathy rats

作　　者：徐自强[1] 何云强[2] 傅红兴[3] 徐艳艳[4] 蔡勇[1] 夏鹏[1] 

机构地区：[1]温州医科大学附属第一医院移植中心,浙江温州325015 [2]温州医科大学附属第一医院外科实验室,浙江温州325015 [3]温州医科大学药学院,浙江温州325035 [4]丽水市中心医院药剂科,浙江丽水323000

出　　处：《温州医科大学学报》2016年第3期163-167,共5页Journal of Wenzhou Medical University

基　　金：浙江省科技厅实验动物平台项目(2013C37006);浙江省自然科学基金资助项目(LQ12H30001;LY16H050007);浙江省卫生厅科研基金资助项目(2012RCB041)

摘　　要：目的:建立微囊化胰岛移植联合肾动脉重建大鼠模型,并观察该模型对糖尿病肾病(DN)大鼠早期肾脏病变的疗效。方法:SD大鼠单次大剂量腹腔注射链脲佐菌素建立糖尿病模型。实验分为3组:1DN组;2微囊组(采用微囊化胰岛腹腔移植);3联合组(进行微囊化胰岛移植联合肾动脉重建)。各组观察4周后,收集24 h内所有尿液,检测尿蛋白总量,并随机检测各组血糖。收集各组肾脏和肾动脉分别进行PAS染色和HE染色,并观察结果。结果:微囊化胰岛移植联合肾动脉重建可以降低大鼠血糖,明显减少尿蛋白。肾脏PAS染色提示联合组和微囊组均可以减轻肾小球硬化,但2组差异无统计学意义。联合组和微囊组肾动脉内膜增厚不显著。结论:微囊化胰岛移植联合肾动脉重建可以显著减轻早期DN病变。Objective： To establish rat＇s model of transplantation of microencapsulated islets combined with renal arterial reconstruction and observe the curative effect of this model on the renal pathology in the early stage for the diabetic nephropathy in rats. Methods： Rat model of diabetes established by injection of a single dose of streptotocin were randomized into diabetic nephropathy（DN） group, microencapsulated islets group and combination group. After 4 weeks, 24 h proteinuria and random blood glucose level of all rats were examined, and pathological changes of the kidney and renal artery were observed under optical microscope. Results： Compared with DN group, blood glucose level and 24 h proteinuria in combination group was significantly lower. Microencapsulated islets group and combination group significantly alleviated glomerulosclerosis compared to DN group. However, there was no significant difference in the glomerulosclerosis between microencapsulated islets group and combination group. In addition, the intimal thickness of renal artery between microencapsulated islets group and combination group was not obvious. Conclusion： Microencapsulated islets combined with renal artery reconstruction can improve early stage renal pathology in DN rats.

关 键 词：胰岛移植 糖尿病肾病 肾小球硬化症 大鼠 

分 类 号：R322.57[医药卫生—人体解剖和组织胚胎学] R587.2[医药卫生—基础医学]