雷公山山区放牧山羊黑素皮质素受体-4mRNA表达的研究  

Study on Melanocortin-4 Receptor mRNA Expression in Goats Grazed in Leigongshan Mountain Area

作　　者：杨家大[1] 吴声榕[1] 潘盛榕[2] 

机构地区：[1]凯里学院,凯里556011 [2]贵州医科大学第二附属医院,凯里556011

出　　处：《中国畜牧兽医》2016年第3期601-607,共7页China Animal Husbandry & Veterinary Medicine

基　　金：黔东南州科技计划项目(黔东南科合J字(2015)001号);贵州省普通本科高校自然科学研究项目(黔教合KY字(2014)236);贵州省科技联合基金项目(黔科合J字LKK(2013)14号);国家自然科学基金(81460572)

摘　　要：为研究放牧山羊黑素皮质素受体-4(melanocortin-4receptor,MC4R)mRNA表达水平的组织差异和品种差异,试验以β-actin基因为内参,应用双标准曲线法实时荧光定量PCR技术检测放牧饲养的黔东南小香羊、贵州白山羊、贵州黑山羊、黔北麻羊和南江黄羊的7个组织MC4R基因mRNA的表达水平。结果表明,5个放牧山羊品种7种组织中均有MC4R mRNA表达,其水平以皮下脂肪和肾脏最高,肝脏其次,肺脏和心脏第三,半膜肌和背最长肌最低。肝脏MC4R mRNA的表达水平无品种差异,皮下脂肪、肾脏、肺脏、心脏、半膜肌和背最长肌MC4R mRNA的表达水平都以贵州白山羊最高。结果提示,放牧山羊MC4R mRNA的表达水平存在组织差异。除肝脏以外,其余组织MC4R mRNA的表达水平存在品种差异。本研究为开展放牧山羊的生长、胴体、肉质等性状的分子标记辅助选择育种奠定了基础。In order to understand the differential expression among tissues or breeds of grazed goat,β-actingene was used as control,the expression levels of melanocortin-4receptor（MC4R）mRNA in 7tissues of Qiandongnan small Xiang goat,Guizhou White goat,Guizhou Black goat,Qianbei Ma goat and Nanjiang Yellow goat were detected by Real-time fluorescence quantitative PCR technique with double standard curve method.The results showed that MC4 R mRNA was expressed in all 7tissues of 5grazed goat breeds,the expression levels of subcutaneous fat and kidney were the highest,the expression levels of liver was the second,the expression levels of lung and heart were the third,and the expression levels of semimembranosus muscle and longissimus muscle were the lowest.There were no differences of the expression level of MC4 RmRNA in liver among different breeds.In subcutaneous fat,kidney,lung,heart,semimembranosus muscle and longissimus muscle,the highest expression levels of MC4 R mRNA were all observed in Guizhou White goat.The results suggested that for grazed goats,the expression levels of MC4 R mRNA existed tissue differences,and except for the liver,it existed breed differences,too.This research laid a basis for molecular marker assisted selection of growth traits,carcass traits,andmeat quality traits of grazed goat.

关 键 词：放牧山羊 黑素皮质素受体-4 基因表达 

分 类 号：S814[农业科学—畜牧学]