银黄清肺胶囊化学成分的LC-ESI-MS/MS分析  

LC-ESI-MS/MS analysis of chemical constituents in Yinhuang Qingfei capsule

作　　者：汪丹[1,2] 蔡甜[3] 吴志军[3] 蒋学华[1] 

机构地区：[1]四川大学华西药学院,四川成都610041 [2]成都大学医学院,四川成都610106 [3]中国科学院成都生物所,四川成都610041

出　　处：《中国测试》2016年第3期36-40,共5页China Measurement & Test

基　　金：国家自然科学基金青年项目(21305137)

摘　　要：研究液质联用技术对银黄清肺胶囊化学成分的分析。对银黄清肺胶囊提取物进行LC-MS分析,确定分子式,鉴定出部分质谱峰的结构。共发现36种化合物,其中有蔗糖、尿苷、2,3-二脱氧尿苷、没食子酸、奎尼酸、新绿原酸、表没食子儿茶素、原儿茶酸、莽草酸、阿魏酸、咖啡酸、反式阿魏酸、犬尿喹啉酸、羟基苯甲酸、4-甲氧基芥子酸、山奈酚、异鼠李素、大黄素18种未在正离子模式下的液质联用文献中报道过。复方中药的有效成分LC-MS检测,正离子模式适用于检测大多数杂原子化合物,负离子模式更适用于含羧基、多羟基的化合物(如酚酸、多酚类、部分黄酮和苷类)的检测,为银黄清肺胶囊进一步质量研究及检测方法选择提供理化依据。To analyze chemical constituents of Yinhuang Qingfei capsules by HPLC-ESI-Q-TOFMS／MS method. The extracts of Yinhuang Qingfei capsules are analyzed with LC-MS method to determine molecular formula and identify the structures of partial spectral peak. 36 compounds are found, including sucrose, uridine, 2,3-deoxyuridine, gallic acid, quinic acid, Neochlorogenic acid,（-）-Epigallocatechin,protocatechuic acid,shikimic acid,ferulic acid,caffeic acid,trans ferulic acid, kynurenic acid, Hydroxybenzoic acid, 4-methoxy-erucic acid, kaempferol,isorhamnetin, Emodin, 18 components that are not reported in HLPC-MS literatures under the positive ion mode.In LC-MS detection of active ingredients in compound traditional Chinese medicine, the positive ion mode can be applied to detect most hetero atomic compounds while the,negative ion mode is more suitable to detect carboxyl-containing and polyhydroxy compounds（such as phenolic acid,polyphenol,flavonoid and glycoside）. This paper has laid a foundation for further studying and testing the quality of Yinhuang Qingfei capsules.

关 键 词：银黄清肺胶囊 LC-ESI-MS 酚酸 黄酮 

分 类 号：TQ336.15[化学工程—橡胶工业]