钙处理对低温胁迫下枇杷幼苗Ca^(2+)-ATPase活性和膜脂过氧化水平的影响  

Effects of calcium on Ca^(2+)-ATPase activity and lipid peroxidation level of loquat seedling under low temperature stress

作　　者：吴锦程[1] 陈宇[1] 吴毕莎[1] 黄玲玲[1] 张伟芳[1] 郑福明[1] 

机构地区：[1]莆田学院环境与生物工程学院,福建莆田351100

出　　处：《西北农林科技大学学报（自然科学版）》2016年第2期121-128,共8页Journal of Northwest A&F University(Natural Science Edition)

基　　金：福建省科技厅重点项目(2011N0028);福建省高校服务海西建设重点项目(2008HX02)

摘　　要：【目的】探讨钙处理对低温胁迫下枇杷叶片Ca2+-ATPase活性和膜脂过氧化的调节机制,为枇杷抗寒性研究提供依据。【方法】采用外源钙(5 mmol/L CaCl2)和钙调素拮抗剂三氟拉嗪(Trifluoperazine,TFP,100mmol/L)处理Hoagland营养液砂培法培养的2年生"早钟6号"枇杷(Eriobotryajaponica Lindl.cv.Zaozhong No.6)容器幼苗,设钙预处理、TFP预处理和先TFP后钙处理,以未做预处理为对照,于-6℃人工气候室进行低温胁迫处理,研究钙处理对低温胁迫下枇杷成活率、CaM含量以及Ca2+-ATPase、CAT、SOD活性和H2O2、MDA含量的影响。【结果】与对照相比,钙处理显著提高了低温胁迫后枇杷幼苗的成活率、叶片细胞CaM含量以及质膜、内质网膜、液泡膜和线粒体膜Ca2+-ATPase活性,并激活了CAT和SOD活性,降低了细胞H2O2和MDA含量;而钙调素拮抗剂TFP对钙处理所诱导的上述生理效应均有抑制作用,削弱了幼苗对低温胁迫的抗氧化能力,加剧了低温对幼苗的伤害。【结论】钙处理提高了枇杷幼苗的抗冻能力,Ca2+-CaM信使系统可能参与了低温胁迫下枇杷抗冻性的诱导和调控。【Objective】The regulation mechanism of calcium on Ca^2＋-ATPase activity and lipid peroxidation of loquat under low temperature stress was studied to provide basis for cold resistance of loquat.【Method】Tow-year-old‘Zaozhong No.6＇loquat（Eriobotryajaponica Lindl.）seedlings planted in containers with sand culture method and Hoagland nutrition solution were treated with 5mmol/L CaCl2and100 mmol/L Trifluoperazine（TFP）.The seedlings with pretreatment with calcium,pretreatment with TFP,pretreatment with TFP after calcium and non-pretreatment（CK）were subjected to low temperature（-6 ℃）stress in a phytotron for 6hto investigate the effects on survival rate,activities of Ca^2＋-ATPase,catalase（CAT）and superoxide dismutase（SOD）,as well as contents of CaM,H2O2 and MDA.【Result】The survival rate of seedlings,CaM content,and Ca^2＋-ATPase activities of plasma membrane,endoplasmic membrane,tonoplast membrane and mitochondrial membrane in leaf cells were markedly enhanced by Ca^2＋treatment.The treatment also stimulated the activities of CAT and SOD while reduced the contents of H2O2 and MDA.The above physiological effects were all inhibited by TFP,which weakened the antioxidantcapacity of loquat seedlings under low temperature stress and aggravated the damage.【Conclusion】Ca^2＋treatment enhanced the cold resistance of loquat seedlings.Signal system of Ca^2＋·CaM may induce and regulate the cold resistance of loquat under low temperature stress.

关 键 词：枇杷 低温胁迫 钙调蛋白 CA^2+-ATPASE 膜脂过氧化 

分 类 号：S667.3[农业科学—果树学] Q946.5[农业科学—园艺学]