紫玉盘内生真菌节菱孢中1个新的异香豆素类化合物  

A new isocoumarin isolated from endophytic fungus Arthrinium sp.A092 derived from medicinal plant Uvaria microcarpa

作　　者：李浩华[1] 周燕燕[1,2] 陈玉婵[1] 孙章华[1] 严寒静[2] 郭晓玲[2] 章卫民[1] 

机构地区：[1]广东省微生物研究所省部共建华南应用微生物国家重点实验室广东省菌种保藏与应用重点实验室广东省微生物应用新技术公共实验室,广东广州510070 [2]广东药学院,广东广州510006

出　　处：《中草药》2016年第3期369-373,共5页Chinese Traditional and Herbal Drugs

基　　金：国家"973"前期专项(2014CB460613);广东省自然科学基金项目(S2012010009773)

摘　　要：目的研究紫玉盘内生真菌节菱孢Arthrinium sp.A092发酵菌丝体的化学成分。方法采用硅胶柱、凝胶柱、HPLC等色谱技术进行分离纯化,根据理化性质和波谱数据进行化合物结构鉴定;以肝癌细胞Hep G-2、乳腺癌细胞MCF-7、大细胞肺癌细胞NCI-H460和神经胶质瘤细胞SF-268为供试细胞株,采用SRB法对化合物进行细胞毒活性测试。结果从节菱孢菌丝体中分离得到10个化合物,分别鉴定为3,4,5-trimethyl-6-methoxy-8-hydroxyisocoumarin（1）、decarboxycitrinone（2）、4-hydroxy-17R-methylincisterol（3）、3-甲氧基-4-羟基苯甲酸（4）、邻苯二甲酸二丁酯（5）、flemingipanic acid（6）、吲哚-3-羧酸（7）、过氧化麦角甾醇（8）、对羟基苯甲酸（9）、4-羟基苯甲醛（10）;化合物3对SF-268和MCF-7的IC50值分别为63.8和57.2μmol/L,化合物8对SF-268、MCF-7和NCI-H460的IC50值分别为50.6、32.3、39.0μmol/L。结论化合物1为新化合物,命名为节菱孢异香豆素A,化合物3～5、7～10均为首次从该属真菌中分离得到;化合物3和8具有一定的细胞毒活性。Objective To study the chemical constituents in fermented mycelium of endophytic fungus Arthrinium sp.A092 from Uvaria microcarpa.Methods The compounds were isolated and purified by chromatography on silica gel,Sephadex-LH20 columns,HPLC,and so on.Their structures were identified on the basis of physicochemical properties and spectroscopic data.All compounds were tested for their cytotoxic activities against four tumor cell lines Hep G-2,MCF-7,NCI-H460,and SF-268.Results Ten compounds were isolated from the mycelial extract and identified as 3,4,5-trimethyl-6-methoxy-8-hydroxyisocoumarin（1）,decarboxycitrinone（2）,4-hydroxy-17R-methylincisterol（3）,4-hydroxy-3-methoxybenzoic acid（4）,dibutyl phthalate（5）,flemingipanic acid（6）,indole-3-carboxy acid（7）,ergosterol peroxide（8）,p-hydroxybenzoic acid（9）,and 4-hydroxybenzal-dehyde（10）.Compounds 3 exhibited the inhibitory activity against SF-268 and MCF-7 with IC50 values of 63.8 and 57.2 μmol/L,respectively.Compounds 8 exhibited the inhibitory activity against SF-268,MCF-7,and NCI-H460 with IC50 values of 50.6,32.3,and 39.0 μmol/L,respectively.Conclusion Compound 1 is a new compound named arthrinisocoumarin A.Compounds 3—5 and 7—10 are isolated from the fungus of Arthrinium sp.for the first time.Compounds 3 and 8 show the moderated cytotoxic activities against SF-268,MCF-7,and NCI-H460.

关 键 词：紫玉盘 内生真菌 节菱孢 节菱孢异香豆素A 邻苯二甲酸二丁酯 过氧化麦角甾醇 4-羟基苯甲醛 细胞毒活性 

分 类 号：R284.1[医药卫生—中药学]