石菖蒲无溶剂微波提取工艺的优化研究及其挥发性成分的GC-MS分析  

Optimization of solvent-free microwave extraction for Acori Tatarinowii Rhizoma and analysis on volatile components by GC-MS

作　　者：王月亮 陈凯[1,2] 李慧[2] 张晓莉[2] 刘宇灵[2] 宋明铭 刘文丛[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]中国中医科学院中药研究所,北京100700

出　　处：《中草药》2016年第3期414-419,共6页Chinese Traditional and Herbal Drugs

基　　金：中国中医科学院自主选题(Z02063);北京市共建项目专项资助

摘　　要：目的石菖蒲挥发油无溶剂微波提取法的工艺优化及其成分分析。方法通过L9(34)正交试验,以α-细辛醚提取量、挥发油体积、3种细辛醚的百分比之和作为评价标准,进行综合评分,优选石菖蒲挥发油无溶剂微波提取法最佳提取工艺;采用GC-MS法对最佳工艺提取的挥发油定性分析,利用峰面积归一化法分析其主要成分,并与水蒸气蒸馏法提取的挥发油对比。结果 2种方法提取的挥发油中成分组成基本相同,石菖蒲中主要挥发性成分为α-细辛醚、β-细辛醚和γ-细辛醚,其在无溶剂微波提取法的百分比为4.12%、55.11%和10.54%,而水蒸气蒸馏提取法则分别为5.39%、47.03%和9.15%。从提取效率来看,无溶剂微波法提油0.235 m L耗时5 min,得α-细辛醚提取量为31.99 mg;水蒸气蒸馏法提油0.175 m L,需要1h,得α-细辛醚提取量为29.09 mg。结论无溶剂微波提取法是一种新的挥发油提取方法,可以缩短提取时间,增加提取效率。Objective To study the solvent-free microwave extraction for oils of Acori Tatarinowii Rhizoma and analyze the volatile components.Methods The paper selected the best technological conditions by L9（34） orthogonal test,with the index of α-asarone,volume of volatile oils,and sum of the percentage,The essential oils in Acori Tatarinowii Rhizoma were analyzed with GC-MS.Results The percentage of volatile oils was calculated according to peak area normalization method.The results showed similar amount of volatile oil components by two methods,and the extraction rates of α-asarone,β-asarone,and γ-asarone accounted for 4.12%,55.11%,and 10.54% on the method of solvent-free microwave extraction,while the steam distillation was 5.39%,47.03%,and 9.15%.To compare with two methods,solvent-free microwave extraction extracted volatile oil 0.235 m L and α-asarone 31.99 mg for 5 min,while steam distillation extracted volatile oil 0.175 m L and α-asarone 29.09 mg for 1 h.The method of solvent-free microwave extraction had the advantage of short reaction time and high yields.Conclusion Solvent-free microwave extraction is a new method with shorter extracting time and better extracting efficiency.

关 键 词：石菖蒲 挥发油 无溶剂微波提取法 水蒸气蒸馏提取法 Α-细辛醚 Β-细辛醚 γ-细辛醚 

分 类 号：R284.2[医药卫生—中药学]