木瓜属药用植物DNA条形码鉴定研究  

Study on DNA barcoding of medicinal plants in Chaenomeles Lindl.

作　　者：张艳艳[1] 齐红 郭庆梅[1] 孙稚颖[1] 周凤琴[1] 李圣波 

机构地区：[1]山东中医药大学,山东济南250355 [2]济南市食品药品检验检测中心,山东济南250001 [3]山东亚特生态技术有限公司,山东临沂266071

出　　处：《中草药》2016年第3期474-479,共6页Chinese Traditional and Herbal Drugs

基　　金：国家科技支撑计划(2011BAI 06 B06);山东省科技发展计划项目(2011GSF 11904)

摘　　要：目的对木瓜属药用植物进行ITS2、psb A-trn H序列分析,为其分子鉴定提供依据。方法对5种木瓜属药用植物的19个样本ITS2序列进行PCR扩增和测序,用MEGA6.0计算其种间、种内的Kimura 2-parameter(K2P)距离,及各序列变异位点并构建系统发育树,并预测ITS2二级结构。结果 ITS2序列间存在明显差异,psb A-trn H序列间也存在稳定差异;构建的系统发育树显示木瓜属的不同基原样本各聚为一支,能很好将5种植物区分,显示出单系性;比较木瓜属5种植物的ITS2二级结构存在明显差异。结论 ITS和psb A-trn H序列可以有效准确鉴别5种木瓜属植物。Objective To provide evidence for the molecular identification of medicinal plants in Chaenomeles Lindl.Methods Nineteen ITS2 and psb A-trn H sequence samples of five species in Chaenomeles Lindl.were PCR amplified and sequenced.The intra-specific and inter-specific K-2P distances were calculated,and cluster analysis was performed using Neighbor-joining（NJ） method by MEGA 6.0.Results The original plant of Chaenomeles Lindl.could be identified by the sequence differences of the ITS2 and psb A-trn H sequences.Different samples of Chaenomeles Lindl.were gathered together and could be distinguished by NJ tree.There were significant differences among the ITS2 secondary structures in five species of Chaenomeles Lindl.Conclusion ITS2 and psb A-trn H are efficient barcodes for the authentication of plants of Chaenomeles Lindl.

关 键 词：木瓜属 DNA条形码 ITS PSBA-TRNH 遗传距离 

分 类 号：R282.12[医药卫生—中药学]