轴突导向因子及其受体在正常及早期胚胎停止发育绒毛组织中的表达及相互关系  

Expression of Netrin-1 and its receptors deleted in colorectal cancer(DCC) and uncoordinated-5-homolog B(UNC5B) in normal and early miscarriage villi and their relationship

作　　者：王珺[1] 李跃琴[1] 史娟娟[1] 宗实[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,沈阳110004

出　　处：《生殖与避孕》2016年第3期171-176,共6页Reproduction and Contraception

基　　金：国家自然科学基金项目(No.81100433);辽宁省科技厅计划项目(2010225031);辽宁省教育厅高等学校科学研究项目(L2010654)

摘　　要：目的:探讨绒毛组织中轴突导向因子(Netrin-1)及其受体结直肠癌缺失基因(deleted in colorectal cancer,DCC)和非协调性分子5同源蛋白B(uncoordinated-5-homolog B,UNC5B)的表达及其与胚胎停止发育的关系。方法:采用免疫组织化学PV法检测20例正常早期妊娠绒毛(对照组)、25例早期胚胎停止发育绒毛(实验组)组织中Netrin-1及其受体DCC、UNC5B的表达情况。结果:Netrin-1在对照组的阳性表达率为95.00%,实验组的阳性表达率为76.00%,组间差异有统计学意义(P<0.01)。DCC在对照组阳性表达率为95.00%,实验组阳性表达率为32.00%,组间差异有统计学意义(P<0.01)。UNC5B在对照组阳性表达率为55.00%,实验组阳性表达率为96.00%,组间差异有统计学意义(P<0.01)。对照组Netrin-1、DCC和UNC5B三者两两间的表达无显著相关性。实验组DCC和Netrin-1的表达间呈显著负相关(r=-0.227,P<0.01),DCC和UNC5B的表达间呈显著正相关(r=0.151,P<0.05)。Netrin-1和UNC5B的表达间无显著相关性(r=-0.065,P=0.303)。结论:Netrin-1及其受体DCC、UNC5B的异常表达可能与早期胚胎停止发育发生有关;三者可能通过协同作用影响早期胚胎发育过程中胎盘血管的形成,导致胚胎停止发育。Objeetive： To explore the clinical significances of the expression of Netrin- 1 and its receptors deleted in colorectal cancer （DCC）, uncoordinated-5-homolog B （UNC5B） with the occurrence of early miscarriage. Methods： Immunohistochemistry PV-6000 two-step method was used to detect the expression of Netrin-1 and its receptors DCC, UNC5B protein in 20 cases of normal early pregnancy villi （control group）, 25 cases of villi from early miscarriage patients （experimental group）. Results： The positive expression rate ofNetrin-1, DCC, UNC5B in control group and experimental group was 95.00% vs 76.00%, 95.00% vs 32.00%, 55.00% vs 96.00%, respectively, with significant differences between the two groups （P〈0.01）. There were no significant correlations among the three proteins in control group. But there was a significantly negative correlation between DCC and Netrin-1 in experimental group （r=-0.227, P〈0.01）, a significantly positive correlation between DCC and UNC5B in experimental group （r=0.151, P〈0.05）. No significant correlation was detected between the expression of UNC5B and Netrin-1 （r=-0.065, P=0.303）. Conclusion： The abnormal expression of Netrin-1 and its receptors DCC and UNC5B might be related to early miscarriage. Those proteins might be involved in the placental vasculogenesis in early embryo development.

关 键 词：早期胚胎停止发育 轴突导向因子(Netrin-1) 结直肠癌缺失基因(DCC) 非协调性分子5同源蛋白B(UNC5B) 免疫组织化学 

分 类 号：R714.2[医药卫生—妇产科学]