慢性乙型肝炎患者PBMCs中ROG、GATA3和T-bet mRNA水平及其意义  

ROG,GATA3 and T-bet mRNA levels in peripheral blood mononuclear cells in patients with chronic hepatitis B

作　　者：张卡[1,2] 舒欣[1,2] 骆海飞[1] 朱凤琴[1] 曹红[1,2] 徐启桓[1,2] 李刚[1,2] 

机构地区：[1]中山大学附属第三医院感染科,广东广州510630 [2]广东省肝脏疾病研究重点实验室,广东广州510630

出　　处：《南方医科大学学报》2016年第2期186-189,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(81500462);广东省医学科研基金(B2012122)~~

摘　　要：目的探讨慢性乙型肝炎患者外周血单个核细胞(PBMC)中转录因子ROG、GATA3和T-bet m RNA水平变化及意义。方法收集135例慢性乙型肝炎患者(轻度45例、中度42例、重度48例)和15例健康志愿者(正常对照组)的外周血,分离单个核细胞,提取总RNA,用实时定量PCR的方法检测ROG、GATA3和T-bet m RNA水平。应用SPSS16.0软件进行统计分析。结果慢性乙型肝炎轻度、中度组和重度组患者外周血PBMCs中T-bet m RNA表达水平均明显高于健康对照组人群,组内比较差异均具有统计学意义(P<0.05)。慢性乙型肝炎重度组患者外周血PBMCs中ROG m RNA表达水平明显高于健康对照组人群、轻度组和中度组患者,差异均有统计学意义(P<0.05)。而轻度组、中度组患者和健康对照组比较差异均无统计学意义(P>0.05)。慢性乙型肝炎中度组和重度组患者外周血PBMCs中GATA3 m RNA表达水平明显高于健康对照组人群和轻度组患者,差异均具有统计学意义(P<0.05);而轻度组和健康对照、中度组患者和重度组比较差异均无统计学意义(P>0.05)。慢性乙型肝炎轻度、中度组和重度组患者T-bet/GATA3比值明显高于健康对照组人群,差异均具有统计学意义(P<0.05);但3组之间比较差异无统计学意义(P>0.05)。ROG表达水平与GATA3和T-bet/GATA3比值均无相关性(P>0.05)。结论 ROG、GATA3和T-bet在慢性乙型肝炎患者外周血PBMCs中表达水平上调,并参与疾病的发生和疾病进展。ROG在纠正和维持Th1/Th2的新平衡状态过程中起重要作用。Objective To investigate the role of ROG, GATA3 and T-bet in the progression of chronic hepatitis B（CHB）. Methods The m RNA levels of ROG, GATA3 and T- bet in peripheral blood mononuclear cells（PBMCs） from 135 patients with CHB（including 45 mild cases, 42 moderate cases, and 48 severe cases） and 15 healthy control subjects were detected by real- time quantitative PCR. Results The levels of T- bet m RNA in the PBMCs were significantly higher in CHB patients than in the healthy controls（P〈0.05）, and also differed significantly between the 3 groups of CHB patients（P〈0.05）. ROG m RNA levels were significantly higher in severe cases of CHB than in the healthy controls and mild and moderate CHB cases（P〈0.05）, but were similar among the latter 3 groups（P〈0.05）. The m RNA level of GATA3 in the PBMCs were significantly higher in moderate and severe CHB cases than in the healthy controls and mild CHB cases（P〈0.05）. The T- bet/GATA3 ratio was significantly greater in the 3 CHB groups than in the control group（P〈0.05） but comparable between the 3 CHB groups（P〉0.05）. ROG levels were not correlated with GATA3 levels or T- bet/GATA3 ratio in the CHB cases. Conclusions The m RNA levels of ROG, GATA3 and T-bet in the PBMCs are obviously up-regulated in CHB patients and these 3 genes may participate in the progression of CHB. ROG plays an important role in correcting and maintaining the new balance of Th1/Th2.

关 键 词：慢性乙型肝炎 转录因子 ROG GATA3 T-BET PBMCS 

分 类 号：R512.62[医药卫生—内科学]