乳化氟碳联合川芎嗪对大鼠肺缺血再灌注损伤的保护作用  

Protective effects of perfluorocarbon combined with ligustrazine against lung ischemia-reperfusion injury in rats

作　　者：郑志雄[1] 彭雪梅[1] 席露[2] 胡冬华[1] 卢春英[1] 

机构地区：[1]暨南大学附属第一医院麻醉科,广东广州510630 [2]广州医科大学第三附属医院麻醉科,广东广州510150

出　　处：《南方医科大学学报》2016年第2期250-254,共5页Journal of Southern Medical University

基　　金：2013年广东省中医药局建设中医药强省科研课题(20132115);2012年广东省高科技发展专项基金资助项目(2060303)

摘　　要：目的探讨乳化氟碳联合川芎嗪对肺缺血再灌注损伤的影响及其机制。方法建立大鼠肺缺血再灌注模型,随机分为对照组(C组)、川芎嗪组(T组)、乳化氟碳组(P组)和乳化氟碳联合川芎嗪组(TP组),每组10只,分别在恢复血供前5 min由尾静脉给药,并在恢复血供3 h后处死动物,取肺脏组织测定丙二醛(MDA)、髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)、肿瘤坏死因子-α(TNF-α)含量,观察肺脏组织病理改变并评分。结果 T组、P组、TP组肺组织MDA、MPO含量显著低于C组(P<0.05),TP组MPO含量显著低于T组和P组(P<0.05);T组、P组、TP组肺组织SOD含量显著高于C组(P<0.05),TP组SOD活性显著高T组和P组(P<0.05);4组动物肺组织TNF-α含量无统计学差异(P>0.05);C组肺组织可见明显水肿、肺泡腔可见红细胞及渗出;T组、P组肺组织未见明显水肿,可见少量红细胞及渗出;TP组肺组织未见明显水肿,可见少量渗出;C组病理积分大于T组、P组及TP组,T组及P组病理积分大于TP组(P<0.05)。结论乳化氟碳及川芎嗪可提高内源性清除氧自由基的能力,抑制中性粒细胞在肺内聚集,减少肺缺血再灌注损伤,两者联合使用效果更好。Objective To investigate the effects of perfluorocarbon and ligustrazine in protecting the lungs against ischemiareperfusion injury in rats. Methds Forty SD rats with ischemia-reperfusion lung injury were randomized equally into control,ligustrazine, perfluorocarbon, and perfluorocarbon plus ligustrazine groups and received the corresponding treatment via the tail vein 5 min before reperfusion. The lung tissues were harvested and the levels of malondialdehyde（MDA）,myeloperoxidase（MPO）, superoxide dismutase（SOD） and tumor necrosis factor- α（TNF- α） were detected 3 h after reperfusion. The pathological changes and pathological scores of the lung tissues were analyzed. Results MDA and MPO levels were significantly lower and SOD activities significantly higher in the lung tissues in the 3 treatment groups than in the control group（P〈0.05）. The rats in the combined treatment group showed a significantly lower MPO level and a significantly higher SOD activity than those treated with ligustrazine or perfluorocarbon alone（P〈0.05）. No significant difference was found in TNF-α levels in the lung tissues among the 4 groups（P〈0.05）. The lung tissues in the control group showed obvious edema and exudation, and the tissues in ligustrazine and perfluorocarbon groups showed no edema but with a few red blood cells and exudation; no edema was found in the combined treatment group with only a small amount of exudation. The pathological scores differed significantly among the 4 groups. Conclusion Perfluorocarbon and ligustrazine, especially in combined use, can promote endogenous oxygen free radical scavenging, decrease peripheral blood proinflammatory cytokines, and inhibit neutrophils filtration in the lungs of rats with ischemia/reperfusion lung injury.

关 键 词：乳化氟碳 川芎嗪 肺缺血再灌注损伤 

分 类 号：R965[医药卫生—药理学]