八氯代二苯并二噁英对小鼠睾丸组织基因表达谱的影响  

Effect of 1, 2, 3, 4, 6, 7, 8, 9-octachlorodibenzo-p-dioxin on testicular gene expression profiles in male mice

作　　者：张伟[1] 王耀峰[1,2] 倪天军[1] 杨志军[1] 

机构地区：[1]新乡医学院基础医学院,河南新乡453003 [2]新乡医学院三全学院,河南新乡453003

出　　处：《南方医科大学学报》2016年第3期316-320,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(81401470);河南省教育厅自然科学研究计划(13B150216;14B150023);新乡医学院高学历人才启动基金(505001)~~

摘　　要：目的探讨八氯代二苯并二噁英(OCDD)对雄性小鼠睾丸基因表达谱改变的影响。方法 20只雄性CBL/6j小鼠,随机分为对照组(玉米油)、OCDD低剂量组(1.25×10-6g/m L)、中剂量组(1.25×10-5g/m L)和高剂量组(1.25×10-4g/m L),每天灌胃1次,连续灌胃30 d后睾丸取材,应用基因芯片技术比较不同浓度OCDD染毒组和对照组雄性小鼠睾丸基因表达差异。结果与对照组相比,低剂量组和对照组差异基因总数1 133个,上调659个,下调474个;中剂量组和对照组差异基因数978个,上调487个,下调491个;高剂量组和对照组的差异基因数895个,上调424个,下调471个。结论亚慢性OCDD暴露后对小鼠的睾丸相关基因表达造成影响,提示睾丸可能是OCDD毒性作用的靶器官。Objective To investigate the effect of 1,2,3,4,6,7,8,9- octachlorodibenzo- p- dioxin（OCDD） on the testicular gene expression profile in the testis of mice. Methods Twenty male C57BL/6j mice were randomly divided into normal control group（fed with maize oil） and 3 OCDD groups treated with OCDD by gavage for 30 days at low-, moderate-, and high doses of 1.25×10-6, 1.25 ×10-5, and 1.25 ×10-4g/m L, respectively（8 m L/kg daily）. The testicular gene expression profiles of the mice were investigated using gene chip technique and compared between OCDD- exposed groups and the control group. Results Compared with the control group, the mice in low-dose OCDD group showed 1133 differentially expressed genes, including659 up-regulated and 474 down-regulated ones; in the moderate-dose OCDD group, 978 genes were differentially expressed,including 487 up- regulated and 491 down- regulated ones; in the high- dose group, 895 genes were differentially expressed,including 424 up- regulated and 471 down- regulated ones. Conclusion The effect of sub- chronic exposure to OCDD on testicular gene expression profiles in male C57BL/6j mice indicates that the testis is probably the target organ of OCDD.

关 键 词：八氯代二苯并二噁英 睾丸 基因表达 基因芯片 

分 类 号：R114[医药卫生—卫生毒理学]