脂多糖诱导体外血小板调亡的作用  

The Influence on Platelet in Vitroapoptosis Induced by Lipopolysaccharide

作　　者：王浩[1] 王兵[2,3] 刘雪淳 王淑娟[2,3] 王勇强[2,3] 

机构地区：[1]天津医科大学一中心临床学院,天津300192 [2]天津市第一中心医院重症医学科,天津300060 [3]天津市第一中心医院急救医学研究所,天津300060

出　　处：《继续医学教育》2016年第3期117-119,共3页Continuing Medical Education

基　　金：卫生部国家临床重点专科建设项目(2011-873);天津市卫生和计划生育委员会重点课题(2014KR07)

摘　　要：目的研究脂多糖(Lipopolysaccharide,LPS)诱导体外血小板凋亡的作用。方法取健康志愿者的新鲜静脉血,分离富血小板血浆(PRP),制备洗涤血小板;把洗涤血小板分为空白对照组、低浓度LPS组、中浓度LPS组、高浓度LPS组、极高浓度LPS组共5组,并加入相应终浓度LPS,各组于室温下孵育30 min;裂解液裂解血小板,并用Western blot检测促凋亡蛋白Bax、Bak和抗凋亡蛋白Bcl-xl的表达情况;采用分析软件进行数据分析。结果低浓度、中浓度、高浓度、极高浓度LPS干预组较对照组促凋亡蛋白Bax、Bak和抗凋亡蛋白Bcl-xl表达明显增多(P<0.05),且呈浓度依赖性。结论 LPS能够诱导体外血小板调亡,并呈现浓度依赖性。Objective This study is intended to investigate the influence on platelet apoptosis induced by Lipopolysaccharide, LPS） in vitro. Methods Drawing fresh venous blood from healthy volunteer to isolate plateletrich plasma（PRP） and prepare washed platelets. The washed platelets were randomly divided into five groups： the group treated by LPS in low concentration, the group treated by LPS in medium concentration, the group treated by LPS in high concentration, the group treated by LPS in highest concentration. Then washed platelets in different groups were treated by LPS in corresponding concentration and incubated for 30 minutes at room temperature. Platelet lysate were prepared by cell lysis buffer and the expression of pro-apoptotic protein Bax, Bak and the expression of antiapoptotic protein Bcl-xl were tested by Western blot. Comparision was carry out by the analysis tool. Results Pro-apoptotic protein Bax, Bak, and the anti-apoptotic protein Bcl-xlex pression were significantly increased in the group of LPS in low, medium, high, highest concentration compared with the control group（P〈0.05）, being in a dose-dependent manner. Conclusion LPS could induce apoptosis of platelet in vitro in dose-dependent manner.

关 键 词：脂多糖 诱导 体外:血小板凋亡 

分 类 号：R33[医药卫生—人体生理学]